精子DNA单双链损伤与精子浓度的关系研究

发布时间：2018-03-04 03:09

本文选题：男性不育　切入点：精子DNA单链损伤　出处：《中国性科学》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究男性不育患者精子浓度与精子DNA单双链损伤的关系,探讨少精症的发生机制。方法:研究对象为120例男性不育患者,年龄25~40岁之间,根据世界卫生标准进行精液常规检测分析精子的浓度、活力等,利用双尾彗星试验分析精子DNA单双链损伤指数。结果:120例男性不育患者根据精子浓度分为三个研究组,即组1(精子浓度10×10~6m L~(-1))、组2(精子浓度20×10~6m L~(-1))和组3(精子浓度20×10~6m L~(-1)),每组40例。三组之间年龄、精子活力及精子畸形率没有统计学差异;组1精子DNA损伤指数(DFI)为(59.48±15.21)%,单链损伤指数(SSB-DFI)为(25.86±11.86)%,SSB占所有损伤精子的比率(SSB-DFI/DFI)为(43.31±13.72)%,双链损伤指数(DSB-DFI)为(31.93±10.21)%,DSB占所有损伤精子的比率(DSB-DFI/DFI)为(53.85±10.27)%;组2精子DNA损伤指数(DFI)为(34.02±1.77)%,单链损伤指数(SSB-DFI)为(26.71±3.45)%,SSB占所有损伤精子的比率(SSB-DFI/DFI)为(78.59±9.78)%,双链损伤指数(DSB-DFI)为(8.37±3.04)%,DSB占所有损伤精子的比率(DSB-DFI/DFI)为(21.40±9.79)%;组3精子DNA损伤指数(DFI)为(20.77±2.43)%,单链损伤指数(SSB-DFI)为(20.44±2.39)%,SSB占所有损伤精子的比率(SSB-DFI/DFI)为(98.44±2.87)%,双链损伤指数(DSB-DFI)为(1.32±0.61)%,DSB占所有损伤精子的比率(DSB-DFI/DFI)为(1.55±2.87)%。三组间SSB-DFI无显著差异(P0.05),SSB-DFI/DFI在组1、组2和组3间随精子浓度升高而逐渐升高(P0.05),DSB-DFI随精子浓度升高而逐渐下降(P0.05),DSB-DFI/DFI随精子浓度升高而逐渐下降(P0.05)。精子SSB-DFI及SSB-DFI/DFI与其他精液常规参数均无相关关系(P0.05),精子DSB-DFI及DSB-DFI/DFI与精子浓度呈负相关(P0.05)。结论:精子DSB-DFI和DSB-DFI/DFI水平越高,精子浓度就越低,精子DNA双链损伤可能是精子浓度低的真正原因。
[Abstract]:Objective: to study the relationship between sperm concentration and single and double strand DNA damage in infertile men and to explore the mechanism of oligozoospermia. Methods: 120 infertile men aged between 25 and 40 years were studied. According to the world health standard, the sperm concentration and sperm motility were analyzed by routine semen test. The sperm DNA single and double strand damage index was analyzed by double tail comet test. Results one hundred and twenty infertile men were divided into three groups according to sperm concentration. There were 40 cases in group 1 (sperm concentration 10 脳 10 6 m / L), group 2 (sperm concentration 20 脳 10 6 m / L) and group 3 (sperm concentration 20 脳 10 6 m / L) in each group (40 cases). There was no significant difference in age, sperm motility and sperm abnormality rate among the three groups. In group 1, the DNA damage index (DSB-DFI) was 59.48 卤15.21, the single strand damage index (SSB-DFI) was 25.86 卤11.86% of all damaged sperm, and the ratio of SSB-DFI / DFI to all damaged sperm was 43.31 卤13.72. The double strand injury index (DSB-DFI) was 31.93 卤10.21. The percentage of DSB-DFIDFI to all damaged spermatozoa was 53.85 卤10.2775; in group 2, the DNA damage index was 34.02 卤1.77g, and the single strand damage index (DSB-DFIDFI) was 53.85 卤10.2775; in group 2, it was 34.02 卤1.77g. The ratio of SSB-DFI to all damaged sperm was 26.71 卤3.45%. The ratio of SSB-DFI / DFI was 78.59 卤9.780.The double strand damage index (DSB-DFI) was 8.37 卤3.040.The ratio of DSB-DFI- DSB-DFII to all damaged sperm was 0.21.40 卤9.790.The DNA damage index of group 3 was 20.77 卤2.75, and the single strand injury index (SSB-DFI) was 20.44 卤2.39. The ratio of SSB-DFI / DFI was 98.44 卤2.870.The double strand damage index (DSB-DFI) was 1.32 卤0.61% of all damaged sperm. The ratio of DSB-DFI / DFI to all damaged sperm was 1.55 卤2.87.There was no significant difference among the three groups in the SSB-DFI of SSB-DFIDFI in group 1, and between groups 2 and 3, the ratio of DSB-DFI to all damaged spermatozoa increased gradually with the increase of sperm concentration. P0.05 DSB-DFI / DFI decreased gradually with the increase of sperm concentration. There was no correlation between sperm SSB-DFI and SSB-DFI/DFI and other normal parameters of semen. There was a negative correlation between sperm DSB-DFI and DSB-DFI/DFI and sperm concentration. Conclusion: the higher the level of DSB-DFI and DSB-DFI/DFI is, the higher the level of DSB-DFI and DSB-DFI/DFI is. The lower the sperm concentration, the more the DNA double strand damage may be the real reason for the low sperm concentration.
【作者单位】：浙江中医药大学第三临床医学院;宁波市药品检验所;浙江中医药大学附属宁波市中医院男科试验室;
【基金】：浙江省医药卫生一般研究计划A类项目(2012KYA172)
【分类号】：R698.2

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