水飞蓟宾对人膀胱癌细胞T24和5637的增殖抑制及凋亡诱导作用

发布时间：2018-03-04 21:12

本文选题：水飞蓟宾　切入点：膀胱肿瘤　出处：《吉林大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：探讨水飞蓟宾(SB)对人膀胱癌细胞T24及5637增值及凋亡的影响，初步阐明其可能的机制。方法：培养人膀胱癌细胞5637和T24，取处于对数生长期的5637和T24细胞，，分别设细胞对照组及25、50、100、200和400μmol·L-1SB实验组，MTT法检测SB对人膀胱癌细胞系T24和5637的增殖抑制作用；细胞划痕实验在倒置显微镜下观察不同浓度SB作用不同时间对人膀胱癌细胞系T245637的侵袭抑制能力；DAPI染色法观察给药后细胞形态的变化；流式细胞术检测并评估SB诱导肿瘤细胞凋亡的能力。结果：MTT检测随着SB剂量的增加和作用时间延长，不同浓度SB组T24和5637细胞存活率显著低于对照组（p＜0.05）；不同剂量SB处理后，各组细胞的迁移明显受到抑制；DAPI染色及流式细胞术检测，不同剂量SB均能够有效诱导T24和5637细胞发生凋亡，凋亡率显著高于对照组（p＜0.05）。结论：SB通过诱导人膀胱癌细胞系T24和5637细胞凋亡从而抑制细胞侵袭及增殖。
[Abstract]:Objective: To investigate the effect of silybin (SB) on the increment and apoptosis of human bladder cancer cell line T24 and 5637, and to elucidate its possible mechanism.
Methods: cultured human bladder cancer cell T24 5637 and 5637, in the logarithmic growth phase and T24 cells, respectively. Cells in the control group and 25,50100200 and 400 mol L-1SB experimental group, inhibition of SB MTT detection method on human bladder cancer cell line T24 and 5637 proliferation; on human bladder cancer cell line T245637 the effects of different concentrations of SB inhibit the invasion ability of different time under inverted microscope, cell scratch assay; DAPI staining was used to observe the cell morphology changes after administration; the ability of flow cytometry to detect and evaluate the SB induced apoptosis in tumor cells.
Results: the detection of MTT with the increase of SB dose and time prolonging, different concentration of SB groups and T24 5637 cell survival rate was significantly lower than the control group (P < 0.05); different doses of SB after treatment, cell migration were significantly inhibited; DAPI staining and flow cytometry, different doses of SB can effectively 5637 and T24 induced cell apoptosis, the apoptosis rate was significantly higher than the control group (P < 0.05).
Conclusion: SB inhibits cell invasion and proliferation by inducing apoptosis of human bladder cancer cell line T24 and 5637 cells.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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