麒麟丸对少弱精子症模型雄性大鼠生殖功能的保护作用研究

发布时间：2018-03-05 06:47

本文选题：麒麟丸　切入点：少弱精子症　出处：《中华男科学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的:研究麒麟丸对雷公藤多苷所致少弱精子症模型雄性大鼠的生殖功能保护作用。方法:取28只雄性SD大鼠,随机分成正常对照组、模型组、麒麟丸低、高剂量组,除正常对照组外,其他各组灌服雷公藤多苷40 mg/(kg·d),同时麒麟丸给药组同日给予不同剂量麒麟丸保护,低剂量组给予麒麟丸1.62 g/(kg·d),高剂量组给予麒麟丸3.24 g/(kg·d)。连续给药30 d后,处死大鼠,称重大鼠睾丸,计算睾丸脏器指数,检测附睾精子浓度、活动率,检测生殖激素水平,评估抗氧化指标,HE切片观察大鼠睾丸组织形态学改变。结果:实验结束后,麒麟丸低、高剂量组精子浓度分别为(14.57±3.95)×10~6/ml、(39.71±11.31)×10~6/ml,精子活力为(3.71±1.11)%、(4.29±1.80)%皆较模型组(4.71±1.25)×10~6/ml、(0.57±0.53)%有明显提高(P0.05);与模型组FSH(1.71±0.52)mIU/ml比较,麒麟丸低、高剂量组FSH下降,分别为(1.49±0.62)mIU/ml、(1.12±0.83)mIU/ml;fT水平较模型组升高(27.27±3.63)nmol/L、(32.80±2.51)nmol/L vs(22.81±2.75)nmol/L,SHBG水平升高(94.83±11.17)nmol/L、(88.05±9.21)nmol/L vs(56.74±8.29)nmol/L,差异皆有显著性(P0.05),但T水平无明显变化(P0.05)。与模型组比较,麒麟丸低、高剂量组SOD水平升高(277.14±15.84)U/ml、(299.60±20.83)U/ml vs(250.04±31.06)U/ml;ROS水平下降(397.61±62.71)IU/ml、(376.84±67.14)IU/ml vs(552.20±58.07)IU/ml(P0.05)。与模型组比较,低、高剂量组HE染色显示麒麟丸能增加少弱精子症大鼠睾丸生精小管内各级生精细胞层次和数量。结论:麒麟丸能明显提高少弱精子症大鼠精子质量,改善生殖激素水平,减轻氧化应激损伤,改善大鼠睾丸组织形态,对生殖系统有较好的保护作用。
[Abstract]:Objective: to study the protective effect of Kirin pill on reproductive function of male rats with oligozoospermia induced by polyglycosides of Tripterygium wilfordii. Methods: 28 male SD rats were randomly divided into normal control group, model group, low dose group and high dose group. In addition to the normal control group, the other groups were treated with Tripterygium wilfordii polyglycoside 40 mg/(kg 路dL, while the Kirin pill group was protected by different doses of Kirin pill on the same day, the low dose group was given the Kirin pill 1.62 g / kg / d, and the high dose group was given the Kirin pill 3.24 g / L / kg 路dl. The rats were killed and called testis. The index of testicular organs was calculated, the sperm concentration, activity rate, reproductive hormone level of epididymis were measured, and the antioxidation index was evaluated to observe the morphological changes of testis in rats. The sperm concentration of Qilin pill was 14.57 卤3.95 脳 10 ~ (-6) / ml ~ (-1) 脳 10 ~ (-6) / ml, and the sperm motility was 3.71 卤1.11 ~ 1.29 卤1.80% respectively. Compared with model group (4.71 卤1.25) 脳 10 ~ (6) / ml (0.57 卤0.53)%, the sperm concentration of Kirin pill was lower than that of model group (FSH(1.71 卤0.52mIUU / ml), and the FSH of high dose group was lower than that of model group (P < 0.05). 1.49 卤0.62nmol / L vs(22.81 卤2.75nmol / L vs(22.81 卤2.75nmol / L vs(22.81 increased 94.83 卤11.17nmol / L vs(56.74 卤8.29nmol / L respectively, the difference was significant (P 0.05 卤8.29nmol / L), but there was no significant change in T level. Compared with the model group, the level of SOD in the Kirin pill group was lower than that in the model group, and the SOD level in the high dose group was 277.14 卤15.84 卤20.83U / ml vs(250.04 卤31.0nmol / L, which was significantly lower than that in the model group. In high dose group, he staining showed that Kirin pill could increase the level and quantity of spermatogenic cells in testicular seminiferous tubules of oligoasthenospermia rats. Conclusion: Kirin pill can obviously improve sperm quality and reproductive hormone level of oligozoospermia rats. It can reduce oxidative stress injury, improve the morphology of testis and protect the reproductive system.
【作者单位】：北京协和医学院研究生院;国家卫生和计划生育委员会男性生殖健康重点实验室国家卫生计生委科学技术研究所/世界卫生组织人类生殖合作研究中心;南方医科大学附属金陵医院/南京军区南京总医院男科;
【分类号】：R285.5;R698.2

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