新多聚糖碳酸氢盐腹膜透析液对人腹膜间皮细胞凋亡及纤维化相关因子的影响

发布时间：2018-03-07 14:19

本文选题：腹膜透析液　切入点：腹膜间皮细胞　出处：《肾脏病与透析肾移植杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:比较新研制的多聚糖碳酸氢盐腹膜透析液(PDF)与常用乳酸盐PDF对人腹膜间皮细胞(HPMC)凋亡及间质纤维化相关因子的影响。方法:体外培养HPMC细胞,使用新型PDF与不同葡萄糖浓度的传统乳酸盐PDF(1.5%、2.5%、4.25%)处理细胞,通过实时荧光定量PCR法检测细胞凋亡因子Bax、胱天蛋白酶3(Caspase-3)及Bcl-2、转化生长因子β1(TGF-β1)、纤维连接蛋白(FN)的mRNA表达量,ELISA法测定细胞分泌TGF-β1量,免疫印迹法检测细胞凋亡蛋白Bax、Caspase-3、抑制凋亡蛋白Bcl-2和FN的表达量。结果:不同PDF刺激HPMC细胞一定时间后,1.5%、2.5%、4.25%传统PDF中葡萄糖浓度越高,Bax、Caspase-3、TGF-β1和FN增高越明显,抑制凋亡因子Bcl-2下降越显著,而新型PDF影响较小。含2.5%、4.25%葡萄糖的PDF刺激后,纤维化相关因子TGF-β1、FN明显高于阴性对照组和新型PDF组(均P0.05)。结论:新型多聚糖碳酸氢盐PDF对腹膜间皮细胞凋亡信号活化低于传统乳酸盐PDF,其腹膜间皮细胞纤维化程度低,有很好的临床应用前景。
[Abstract]:Objective: to compare the effects of polycarbohydrate peritoneal dialysate (PDF) and lactate PDF on apoptosis and interstitial fibrosis related factors of human peritoneal mesothelial cells. Methods: HPMC cells were cultured in vitro. The cells were treated with a new type of PDF and traditional lactate with different glucose concentrations. The expression of apoptosis factor (Bax, cystatin 3 Caspase-3) and Bcl-2, transforming growth factor 尾 1 (TGF- 尾 1) and fibronectin (FN) were detected by real-time fluorescence quantitative PCR method. The level of TGF- 尾 1 secreted by cells was determined by Elisa. The expression of apoptosis protein Bcl-2 and FN was detected by Western blotting. Results: the higher the glucose concentration of Baxia Caspase-3 TGF- 尾 1 and FN in 4.25% traditional PDF, the higher the concentration of BaxCaspase-3 TGF- 尾 1 and FN in HPMC cells stimulated by different PDF for a certain time. The decrease of apoptosis factor Bcl-2 was more significant, but the effect of new type PDF was less. After the stimulation of PDF containing 2.5% glucose, 4. 25% glucose, Fibrosis-related factor TGF- 尾 1 FN was significantly higher than that in negative control group and new type PDF group (all P0.050.Conclusion: the activation of apoptotic signal in peritoneal mesothelial cells by new type polycarboxylate PDF is lower than that of traditional lactate PDF, and the degree of peritoneal mesothelial cell fibrosis is low. It has a good prospect of clinical application.
【作者单位】：暨南大学附属第一医院肾脏内科;暨南大学附属第一医院门诊部;
【基金】：广东高校工程技术研究(开发)中心计划(GCZX-A1104)
【分类号】：R692

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