高糖环境下缬沙坦对肾小管上皮细胞-间充质转化过程中骨膜蛋白、转录激活因子1的影响

发布时间：2018-03-08 12:53

  本文选题：缬沙坦　切入点：肾小管上皮HK-细胞　出处：《中国药房》2016年13期 　论文类型：期刊论文

【摘要】：目的:研究在高糖环境下缬沙坦对肾小管上皮细胞-间充质转化(EMT)过程中骨膜蛋白(Periostin)、转录激活因子1(AP-1)的影响。方法:体外高糖环境下培养人肾小管上皮细胞(HK-2细胞),分为空白对照组和缬沙坦高、中、低剂量组(10~(-3)、10~(-4)、10~(-5)mol/L),作用48 h;另设正常对照组。观察各组HK-2细胞形态学变化,检测各组HK-2细胞活力(OD值),细胞中Ⅲ型胶原含量、AP-1蛋白表达和Periostin、AP-1 mRNA表达。结果:与正常对照组比较,其余各组HK-2细胞部分细胞间隙增大并与附近的细胞脱离接触,细胞分裂旺盛,细胞活力、Ⅲ型胶原含量、AP-1蛋白表达和Periostin、AP-1 mRNA表达均增强(P0.05)。与空白对照组比较,缬沙坦高、中、低剂量组细胞活力、Ⅲ型胶原含量、AP-1蛋白表达和Periostin、AP-1 mRNA表达均更低(P0.05),其中缬沙坦中剂量组作用最明显(P0.01)。结论:体外高糖环境下可促进HK-2细胞EMT过程;缬沙坦可通过抑制AP-1的过表达进而下调转分化的肾小管上皮细胞中Periostin蛋白表达,从而减缓EMT过程。
[Abstract]:Objective: to study the effect of valsartan on the expression of periostein-Periostin1 (transcriptional activator 1) in the process of renal tubular epithelial cell-mesenchymal transformation (EMTT). Methods: human renal tubular epithelial cells (HK-2) were cultured in high glucose environment in vitro. Cystonia, divided into blank control group and valsartan high, In the low dose group, the cells of HK-2 were observed in morphologic changes, OD value of HK-2 cells, and the expression of AP-1 protein and PeriostinAP-1 mRNA in the cells. Results: compared with the normal control group, the expression of AP-1 protein and PeriostinAP-1 mRNA in the cells were compared with those in the normal control group, and the morphological changes of the HK-2 cells in each group were observed, and the OD value of HK-2 cells in each group was measured, and the expression of AP-1 protein and PeriostinAP-1 mRNA in the cells were compared with those in the normal control group. In other groups, the gap of some HK-2 cells was enlarged and disconnected from the nearby cells. The cell division, cell viability, the expression of AP-1 protein and the expression of Periostin AP-1 mRNA in type 鈪，

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