循环miR-152对前列腺癌生化复发早期预测的研究

发布时间：2018-03-08 15:27

本文选题：前列腺癌　切入点：微小RNA　出处：《中华男科学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:探讨循环微小RNA 152(miR-152)对前列腺癌生化复发早期预测的价值。方法:收集将要进行前列腺癌手术的患者66例(生化复发组:35例,术后2年内生化复发;未生化复发组:31例,术后2年内未生化复发),另择年龄相匹配的31例健康男性作为健康对照组。用qRT-PCR法检测前列腺癌患者和健康对照组的循环miR-152表达水平。应用ROC曲线分析循环miR-152对前列腺癌早期生化复发的诊断价值。分析复发组循环miR-152表达水平与临床病理参数(年龄、术前PSA水平、Gleason积分、临床分期、骨转移)之间的相关性。结果:循环miR-152在前列腺癌术后患者(未生化复发及生化复发)与健康对照组的表达差异有统计学意义(t=-5.212,P=0.001)。前列腺癌术后生化复发组患者循环miR-152的表达量较未生化复发组患者低,差异有统计学意义(t=-5.727,P=0.001)。循环miR-152对前列腺癌术后生化复发早期预测的ROC曲线下面积(AUC)为0.906(95%CI,0.809~0.964),灵敏度和特异度分别为91.4%、80.6%。循环miR-152表达水平与Gleason积分、临床分期、有无生化复发和骨转移相关(P均0.05);随着Gleason积分、临床分期的增加,循环miR-152的表达水平下降。但miR-152表达量与患者年龄、术前PSA无关(P均0.05)。结论:循环miR-152表达水平在前列腺癌术后生化复发患者中显著降低,可能成为前列腺癌生化复发早期预测的分子标志物。
[Abstract]:Objective: to investigate the value of circulating minimal RNA 152miR-152 in early prediction of biochemical recurrence of prostate cancer. Methods: 66 patients (35 cases of biochemical recurrence group, 35 cases of biochemical recurrence group and 31 cases of non-biochemical recurrence group) were collected. The circulating miR-152 expression in prostate cancer patients and healthy control group was detected by qRT-PCR method. The circulating miR-152 expression was analyzed by ROC curve. Diagnostic value of early biochemical recurrence of prostate cancer: analysis of circulating miR-152 expression and clinicopathologic parameters (age, age). Preoperative PSA level, Gleason score, clinical stage, Results: the expression of circulating miR-152 in postoperative patients with prostate cancer was significantly different from that in healthy control group. The expression of cyclic miR-152 was lower than that of non-biochemically relapsed patients. The area under the ROC curve of circulating miR-152 for the early prediction of postoperative biochemical recurrence of prostate cancer was 0.906 ~ 95CII 0.809 ~ 0.964U, the sensitivity and specificity were 91.4 ~ 80.6.The circulating miR-152 expression level, Gleason integral, clinical stage, and so on. The expression of circulating miR-152 decreased with the increase of Gleason score and clinical stage, but the expression of miR-152 was correlated with the age of the patients. Conclusion: the expression of circulating miR-152 was significantly decreased in patients with postoperative recurrence of prostate cancer and might be a molecular marker for early prediction of biochemical recurrence of prostate cancer.
【作者单位】：温州医科大学检验医学院;温州医科大学生命科学院;宁波市第二医院检验科;宁波市第二医院泌尿外科;
【基金】：浙江省医药卫生科技计划(2014KYB233) 宁波市自然科学基金(2012A610194) 宁波市第二医院华美研究基金(2016HMKY08)~~
【分类号】：R737.25

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