骨髓间充质干细胞不同途径输注对阿霉素肾病大鼠AQP1、AQP2表达的影响

发布时间：2018-03-09 05:06

本文选题：骨髓间充质干细胞　切入点：移植途径　出处：《介入放射学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的评估骨髓间充质干细胞(MSC)分别通过肾动脉和尾静脉移植方式对大鼠慢性肾病(CKD)模型肾损伤修复的疗效,比较不同移植方式对CKD大鼠水通道蛋白(AQP)1、AQP2表达差异的影响。方法实验取50只大鼠,2只制备骨髓MSC;36只经尾静脉输注阿霉素构建CKD模型,随机分为阿霉素CKD(A-C)组(n=12)、骨髓MSC经肾动脉输注(M-A)组(n=12)、骨髓MSC经尾静脉输注(M-V)组(n=12);另12只为正常对照(N)组。末次骨髓MSC移植隔1周后检测大鼠24 h尿量、24 h尿蛋白定量、血清钠及血清白蛋白,免疫组化检测肾组织AQP1、AQP2表达。结果与A-C组相比,M-V组、M-A组大鼠血清白蛋白及24 h尿量均增高(P0.05),24 h尿蛋白及血清钠水平均降低(P0.05),M-A组24 h尿蛋白较M-V组降低更明显(P0.05)。M-V组、M-A组大鼠肾脏AQP1、AQP2表达均较A-C组降低(P0.05),M-V组、M-A组间差异无统计学意义(P0.05)。结论骨髓MSC移植可增加血清白蛋白,降低尿蛋白、血清钠和肾实质细胞内AQP1、AQP2表达,对修复阿霉素CKD大鼠肾损伤有作用。在一定时间内肾动脉途径移植效果优于外周静脉途径,但两种移植途径的效果差异与AQP1、AQP2表达无明显关联。
[Abstract]:Objective to evaluate the effect of bone marrow mesenchymal stem cell (MSCs) transplantation via renal artery and caudal vein on renal injury and repair of chronic nephropathy model in rats. To compare the effect of different transplantation methods on the expression of aquaporin aquaporin-1 (AQP-1) AQP2 in CKD rats. Methods the CKD model was established by injecting adriamycin into bone marrow of 50 rats and 2 rats. They were randomly divided into two groups: adriamycin CKD A-C group (n = 12), bone marrow MSC via renal artery infusion (M-A) group (n = 12), bone marrow MSC via tail vein infusion group (n = 12) and normal control group (n = 12). The urine quantity of 24 h urine and 24 h urine protein were measured after the last bone marrow MSC transplantation. Serum sodium and serum albumin, Results compared with A-C group, the serum albumin and 24 h urine volume of rats in M-V group were higher than those in group A-C. The levels of urinary protein and sodium in 24 h urine were significantly lower in M-A group than those in M-V group, and those in M-V group were significantly lower than those in M-V group. The expression of AQP1AQP2 in kidney of rats in M-A group was lower than that in A-C group. There was no significant difference between M-V group and M-V group. Conclusion Bone marrow MSC transplantation can increase serum albumin. Reducing the expression of urinary protein, serum sodium and AQP1- AQP2 in renal parenchyma cells can repair renal injury in adriamycin CKD rats. However, there was no significant correlation between the effect of the two transplants and the expression of AQP2.
【作者单位】：昆明医科大学第二附属医院放射科;
【基金】：云南省科技厅-昆明医科大学应用基础研究联合专项项目(2017FE467) 云南省中青年学术和技术带头人后备人才项目(2017HB037)
【分类号】：R692

【相似文献】