肾移植患者尿液Smad2,4,7蛋白检测分析早期发现慢性移植肾肾病

发布时间：2018-03-10 01:11

本文选题：转化生长因子β　切入点：smad蛋白　出处：《宁波大学》2014年硕士论文　论文类型：学位论文

【摘要】：摘要：目的：探讨肾移植后患者尿中smad2，4，7蛋白表达与慢性移植肾肾病（CAN）的关系，以期将检测尿液中Smads蛋白表达作为一个无创检测指标用于临床。方法：实验分为2组，，CAN组：肾移植术后发生CAN的患者30例；对照组：肾移植术后未发生CAN患者30例，留取各组研究对象的早晨中段尿液。用酶联免疫技术（Elisa）检测CAN组和对照组患者尿液中的Smad2、Smad4、Smad7的蛋白表达，用Real-Time PCR检测CAN组和对照组患者尿液中的Smad2、Smad4、Smad7的mRNA表达。结果：经Elisa检测，CAN组患者尿液样本中Smad2、 Smad4、Smad7与非CAN组患者水平的表达存在差异（p0.05）。RT-PCR结果显示，CAN组患者尿液样本中Smad2、Smad4的mRNA表达均明显高于非CAN组患者，而非CAN组患者尿液样本中Smad7的mRNA表达量显著高于CAN组患者。（p0.05，具有统计学意义）。结论：通过我们的研究发现，CAN患者尿液中Smad2、Smad4的表达及含量均显著高于非CAN患者组，而Smad7的表达及含量则显著低于另一组，因此我们认为利用尿液Smad2、Smad4及Smad7联合诊断CAN具有一定的临床意义。这为我们找到了一个可以利用并且比较便捷的无创诊断慢性移植肾肾病的方法，从而为临床医生在临床诊断中提供了非常具有价值的参考资料，也为门诊动态观察和随访肾移植受者提供了一个可靠的生物学指标。
[Abstract]:Abstract: objective: to investigate the relationship between the expression of Smad2O4M7 protein in urine and chronic renal allograft nephropathy (CAN) after renal transplantation. Methods: the patients were divided into two groups: 30 patients with CAN after renal transplantation, 30 patients without CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients with CAN after renal transplantation, 30 patients without CAN after renal transplantation. The urine samples of each group were collected in the middle of the morning. The protein expression of Smad2 and Smad4 Smad7 in the urine of CAN group and control group was detected by enzyme-linked immunosorbent assay (Elisa). Real-Time PCR was used to detect the mRNA expression of Smad2, Smad4, Smad4, Smad7 in the urine of CAN group and control group. Results: there was a difference in the expression of Smad2, Smad4, Smad4, Smad7 between the CAN group and the non-#en5# group by Elisa. The results of RT-PCR showed that there was significant difference in urine samples of can group. The mRNA expression of Smad2 + Smad4 was significantly higher than that of non-#en1# patients. However, the mRNA expression of Smad7 in urine samples of patients without CAN was significantly higher than that of patients with CAN (P 0.05). Conclusion: the expression and content of Smad2 Smad4 in urine of patients with can are significantly higher than those of patients without CAN. The expression and content of Smad7 were significantly lower than those in the other group. Therefore, we think that the use of urine Smad2 Smad4 combined with Smad7 in the diagnosis of CAN is of certain clinical significance, which provides us with a more convenient and noninvasive method for the diagnosis of chronic renal allograft nephropathy. It provides a valuable reference for clinicians in clinical diagnosis and a reliable biological index for dynamic observation and follow-up of renal transplant recipients in outpatient clinic.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R699.2

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