左卡尼汀抑制尿毒症血清诱导的红细胞衰亡

发布时间：2018-03-12 18:45

本文选题：尿毒症血清　切入点：红细胞衰亡　出处：《中国病理生理杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的:探讨左卡尼汀对尿毒症患者红细胞的保护作用及机制。方法:将2%健康人红细胞悬液在以下3组不同的体外培养液中孵育:对照组(C组)、尿毒症血清组(U组)和尿毒症血清+左卡尼汀组(L组)。分别在孵育24和48 h时,留取红细胞,使用流式细胞术检测红细胞衰亡(用磷脂酰丝氨酸表达率代表红细胞衰亡)和红细胞活性氧簇(ROS)的含量,并采用酶联免疫法检测红细胞谷胱甘肽(GSH)的含量。结果:红细胞衰亡随孵育时间延长而增加,U组较C组明显增加,L组较U组明显降低。U组的红细胞ROS生成较C组明显增加,而红细胞GSH生成明显减少。L组的红细胞ROS生成较U组明显减少,而GSH生成明显增加。结论:尿毒症血清诱导正常红细胞加速衰亡,且衰亡率具有时间依赖性。而左卡尼汀可以抑制尿毒症血清诱导的红细胞衰亡,其机制可能与抗氧化有关。
[Abstract]:Objective: to investigate the protective effect and mechanism of levacarnitine on erythrocytes of uremic patients. Methods: the erythrocyte suspension of 2% healthy persons was incubated in the following three groups: control group C, uremia serum group. Group A and group L were incubated for 24 h and 48 h, respectively, and the serum levocarnitine in uremia group was treated with L-carnitine for 24 h and 48 h, respectively. The contents of erythrocyte death (erythrocyte decayed by phosphatidyl serine expression rate) and erythrocyte reactive oxygen species (Ros) were detected by flow cytometry. The erythrocyte glutathione glutathione (GSH) content was detected by enzyme linked immunosorbent assay (Elisa). Results: with the increase of incubation time, the erythrocyte ROS production in group U was significantly higher than that in group C, and the ROS production in group U was significantly lower than that in group U. However, erythrocyte ROS production was significantly decreased and GSH production was significantly increased in group .L, conclusion: serum from uremia induced accelerated death of normal erythrocytes. Moreover, the decay rate is time-dependent, and levocarnitine can inhibit the erythrocyte decay induced by uremic serum, which may be related to antioxidation.
【作者单位】：山东大学第二医院血液净化科;济南市委门诊部;
【分类号】：R692.5

【共引文献】