扩增活化的自体淋巴细胞对肾母细胞瘤的影响

发布时间：2018-03-17 11:22

本文选题：肾母细胞瘤　切入点：扩增活化的自体淋巴细胞　出处：《广东医学》2017年S1期 　论文类型：期刊论文

【摘要】：目的初步探讨扩增活化的自体淋巴细胞(EAAL)对肾母细胞瘤移植瘤生长的影响。方法 15只裸鼠随机分为低、高剂量组和阴性对照组,皮下种植人肾母细胞瘤组织。小鼠瘤体模型建立后,分别经尾静脉输注低剂量EAAL(1.7×10~5,0.3 m L)、高剂量EAAL(15×10~5,0.3 m L)、阴性对照组(注射生理盐水0.3 m L),对比观察肿瘤生长变化及裸鼠生存状况。结果动物实验结果表明:注射EAAL细胞后,各组间裸鼠肿瘤直径变化有差异,低、高剂量组较阴性对照组裸鼠肿瘤直径缩小[(0.97±0.10)cm、(0.64±0.07)cm vs(1.33±0.09)cm;P0.05]。瘤体质量方面,低、高剂量组亦较阴性对照组裸鼠减轻[(0.96±0.09)g、(0.62±0.05)g vs(1.20±0.11)g;P0.05],且EAAL剂量越高对肿瘤的抑制作用越明显。结论 EAAL细胞治疗对裸鼠肾母细胞瘤移植瘤的生长可能有抑制作用。
[Abstract]:Objective to investigate the effect of expanded activated autologous lymphocyte (EAALL) on the growth of nephroblastoma transplantation tumor. Methods Fifteen nude mice were randomly divided into low dose, high dose group and negative control group. Human nephroblastoma tissue was implanted subcutaneously. After the establishment of mouse tumor model, Low dose EAAL(1.7 脳 10 0. 3 ml / L, high dose EAAL(15 脳 10 0. 0 3 mL / L and negative control group (saline 0. 3 mL / L) were injected respectively through tail vein to observe tumor growth and survival status of nude mice. Results: after EAAL cells were injected, the tumor growth and survival status of nude mice were observed. The tumor diameter of nude mice in the high dose group was smaller than that in the negative control group [0.64 卤0.07 cm vs(1.33 卤0.09 cm]. The tumor mass of the high dose group was lower than that of the negative control group. The high dose group was also less than the negative control group [0.96 卤0.09 vs(1.20 卤0.62 卤0.05g vs(1.20 卤0.11g (P0.05)], and the higher the dose of EAAL was, the more obvious the inhibitory effect on the tumor was. Conclusion EAAL cell therapy may have an inhibitory effect on the growth of transplanted nephroblastoma in nude mice.
【作者单位】：广西医科大学第一附属医院小儿外科;
【基金】：广西壮族自治区教育厅高校科研项目(编号:YB2014071)
【分类号】：R737.11

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