雷公藤多苷对2型糖尿病肾脏疾病大鼠肾脏保护作用的研究

发布时间：2018-03-22 22:31

本文选题：糖尿病肾脏疾病　切入点：核因子κB受体活化因子/核因子κB受体活化因子配体　出处：《中国糖尿病杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的观察雷公藤多苷对STZ诱导的糖尿病肾脏疾病(DKD)大鼠肾脏中细胞核因子κB受体活化因子/核因子κB受体活化因子配体(RANK/RANKL)的表达变化,探讨其保护肾脏的机制。方法采用高糖高脂喂养加STZ腹腔注射法建立T2DM动物模型,造模成功后随机分为DKD模型组(DKD,n=8)和雷公藤多苷组(DT,n=8),另选健康大鼠作为正常对照组(NC,n=8)。DT组予雷公藤多苷50mg/(kg·d)剂量灌胃,NC组和DN组每日予等量生理盐水灌胃。12周后处死大鼠,检测FPG、FIns、UAlb、BUN、Scr、Ucr,计算Ccr。PAS染色观察肾脏病理改变,免疫组织化学法检测肾脏中RANK、RANKL的表达分布,Western blot检测RANK、RANKL、Nephrin的蛋白表达。结果与NC组比较,DKD组FPG、FIns、UAlb、Ccr、BUN在给药12周末表达均升高,肾脏中RANK[(0.27±0.05)vs(0.68±0.11)]、RANKL[(0.23±0.07)vs(0.62±0.08)]蛋白表达增加,而Nephrin表达水平下降(P0.01);与DN组比较,DT组上述生化指标均改善,肾脏病理改变减轻;RANK[(0.45±0.09)vs(0.68±0.11)]、RANKL[(0.39±0.06)vs(0.62±0.08)],而Nephrin蛋白表达增多(P0.01)。结论雷公藤多苷可以抑制RANK/RANKL的表达,减轻T2DM大鼠肾脏病理改变,减少蛋白尿,起到肾脏保护作用。
[Abstract]:Objective to observe the effect of tripterygium wilfordii on the expression of nuclear factor 魏 B receptor activating factor / nuclear factor 魏 B receptor activating factor ligand RANKR / RANKL in the kidney of diabetic nephropathy rats induced by STZ. Methods the animal model of T2DM was established by high glucose and high fat feeding plus STZ intraperitoneal injection. After the model was successfully established, the rats were randomly divided into two groups: DKD model group (DKDN 8) and tripterygium wilfordii polyglycoside group (TDC) and tripterygium tripterygii (50mg/(kg d) group. The rats in the control group were given the same amount of normal saline daily for 12 weeks, and the rats in the control group were killed by intragastric administration of tripterygium tripterygii polyglycoside (50mg/(kg d) daily for 12 weeks. Ccr.PAS staining was used to observe the pathological changes of kidney, and immunohistochemical method was used to detect the distribution of RANKL in kidney. The protein expression of RANKL nephrin was detected by Western blot. Results compared with NC group, the expression of Ccr.PAS Ins UAlbCcrbun in DKD group was increased at the end of 12 weeks. In kidney, the expression of RANK [0.27 卤0.05)vs(0.68 卤0.11] RANKL [0.23 卤0.07)vs(0.62 卤0.08] increased, but the expression of Nephrin decreased, compared with DN group, the above biochemical indexes were improved, the pathological changes of kidney alleviated RANKL [0.39 卤0.06)vs(0.62 卤0.08], while the expression of Nephrin protein increased P0.01.Conclusion Tripterygium wilfordii can inhibit the expression of RANK/RANKL. The renal pathological changes and proteinuria were alleviated in T2DM rats.
【作者单位】：青岛大学附属医院肾内科;青岛海慈医疗集团肾病科;
【分类号】：R587.2;R692.9

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