NFKB1基因启动子多态性与中国汉族人膀胱癌的关系

发布时间：2018-03-25 17:18

本文选题：膀胱癌　切入点：基因多态性　出处：《南京医科大学》2014年硕士论文

【摘要】：目的：研究NFKB1基因启动子-94ins/del ATTG与中国汉族人膀胱癌的相关性。方法：采用Taqman-MGB方法检测609例中国汉族人群膀胱癌患者与640例健康对照者NFKB1基因启动子-94ins/del ATTG基因型。运用Logistic回归模型评价单核苷酸多态性与膀胱癌危险因素之间的相关性。采用实时定量聚合酶链反应分析NFKB1mRNA的表达。结果：携带del/del基因型的个体与携带ins/ins或ins/del基因型的个体相比,患膀胱癌的风险增加1.92倍(校正OR=1.92,95%CI=1.42-2.59).在分层分析中,携带del/del基因型的个体与携带ins/ins或ins/del基因型的个体相比患膀胱癌风险明显增高的人群有：年龄超过65岁(OR=2.37,95%CI=1.52-3.70),男性(OR=1.97,95%CI=1.40-2.79),有肿瘤家族史(OR=3.59,95%CI=1.19-10.9),风险分别增加2.37倍、1.97倍、3.59倍。此外,携带del/del基因型的个体与携带ins/ins或ins/del基因型的个体相比,患非肌层浸润性膀胱癌(OR=2.07,95%CI=1.51-2.85)、膀胱癌I级(OR=2.40,95%CI=1.68-3.43)、单发膀胱癌(OR=2.04,95%CI=1.48-2.82)、较小膀胱肿瘤(OR=2.10,95%CI=1.51-2.92)的风险更高,风险分别增加2.07倍、2.40倍、2.04倍。NFKB1mRNA的表达,在携带插入型纯合子基因型的患者膀胱癌组织中比携带缺失型等位基因型患者膀胱癌组织中高。结论：NFKB1基因启动子-94ins/del ATTG基因多态性可能是中国汉族人膀胱癌的致病因素。
[Abstract]:Aim: to study the association between NFKB1 gene promoter -94insdel ATTG and bladder cancer in Chinese Han nationality. Methods: the NFKB1 gene promoter -94ins-del ATTG genotype was detected by Taqman-MGB in 609 patients with bladder cancer in Chinese Han nationality and 640 healthy controls. Logistic regression model was used to evaluate the relationship between single nucleotide polymorphisms and risk factors of bladder cancer. Real-time quantitative polymerase chain reaction (PCR) was used to analyze the expression of NFKB1mRNA. Results: the risk of bladder cancer in individuals with del/del genotype was 1.92 times higher than that with ins/ins or ins/del genotype. The individuals with del/del genotype had significantly higher risk of bladder cancer compared with those with ins/ins or ins/del genotype: the risk of bladder cancer was 2.37%, 1.57% and 3.70%, 1.97% and 1.40-2.79%, respectively, and the risk of bladder cancer was 2.37 times 1.97 times 3.59 times higher than that of the individuals with ins/ins or ins/del genotype. In addition, the risk was 2.37 times 1.97 times and 3.59 times higher than that of the individuals with ins/ins or ins/del genotypes. In addition, the risk of bladder cancer was increased by 2.37 times or 3.59 times respectively, and the incidence of cancer was 1.595% 1.19-10.9% higher than that of the individuals with ins/ins or ins/del genotype. Compared with those with ins/ins or ins/del genotypes, individuals with del/del genotype had a higher risk of developing non-myometrial invasive bladder cancer, OR2.07-2.85, OR2.4095CI1.68-3.43C, single bladder cancer 2.0495CI1.48-2.82, and small bladder cancer 2.1095CIT 1.51-2.92. The risk was 2.07 times 2.40 times 2.04 times higher than that of 2.1095 CI 1.51-2.92, respectively, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/del genotype or ins/ins genotype, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/ins or ins/del genotypes, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/ins or ins/del genotypes, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/ins or ins/del genotypes, respectively. More bladder cancer tissues were found in patients with insertion homozygous genotypes than those with deletion alleles. Conclusion the gene polymorphisms of -94 ins / del ATTG gene of the 10% NFKB1 gene may be the pathogenetic factor of bladder cancer in Chinese Han people.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

【参考文献】