低氧对前列腺间质细胞活性氧、低氧诱导因子-1α、雄激素受体表达的影响及依达拉奉的干预作用

发布时间：2018-03-28 06:37

本文选题：低氧　切入点：活性氧　出处：《中国老年学杂志》2017年24期

【摘要】：目的探讨低氧环境下活性氧(ROS)在前列腺增生中的作用及依达拉奉对其的干预作用。方法流式细胞仪检测前列腺增生组织及原代培养细胞中ROS的表达,低氧条件下观察前列腺间质细胞ROS及生长变化,荧光定量PCR法检测低氧诱导因子(HIF)-1α、雄激素受体(AR)的变化。低氧条件下用依达拉奉干预后,检测细胞生长及ROS、HIF-1α、AR的变化。结果前列腺增生组织及3%O_2条件下细胞ROS均明显升高(P0.01),3%O_2条件下细胞增殖升高(P0.01);HIF-1α、AR表达上调(均P0.05);在3%O_2条件下依达拉奉干预后,ROS明显降低(P0.01),HIF-1α明显下调(P0.05);细胞增殖明显下降(P0.01)。结论低氧及低氧环境刺激产生的ROS在前列腺增生中可能起关键作用。
[Abstract]:Objective to investigate the role of reactive oxygen species (Ros) in prostatic hyperplasia (BPH) and the effect of Edaravone on prostatic hyperplasia (BPH). Methods flow cytometry was used to detect the expression of ROS in prostatic hyperplasia tissues and primary cultured cells. The changes of ROS and growth of prostatic stromal cells were observed under hypoxic conditions, and the changes of HIF-1 伪, androgen receptor ARA were detected by fluorescence quantitative PCR. Cell growth and the changes of HIF-1 伪 -AR in prostatic hyperplasia and 3%O_2 were detected. Results the expression of HIF-1 伪 -AR in prostatic hyperplasia and 3%O_2 was significantly increased under the condition of P0.01T3O _ 2. The expression of HIF-1 伪 -tir was up-regulated (all P0.05A), and Ros significantly decreased the expression of HIF-1 伪 (P0.01HIF-1 伪) after the intervention of Edaravone under the condition of 3%O_2. P0. 05 and P0. 01 were significantly down-regulated. Conclusion ROS induced by hypoxia and hypoxia may play a key role in prostatic hyperplasia.
【作者单位】：青海省人民医院分子病理实验室;青海大学附属医院泌尿外科;
【基金】：青海省自然科学基金(No.2012-Z-939Q)
【分类号】：R697.3

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