脑组织特异性血管生成抑制因子1（BAI1）在肾癌中的表达及临床相关性研究

发布时间：2018-03-29 04:27

本文选题：肾癌　切入点：透明细胞癌　出处：《兰州大学》2014年硕士论文

【摘要】：肾癌,又称肾细胞癌(renal cell carcinoma, RCC),其发病率占全身恶性肿瘤的3%,在病理上75%表现为透明细胞癌,不同病理类型的肾癌具有不同的临床特点、治疗方案和预后。血尿、疼痛、肿块称为肾癌的三联征。临床上有30%-50%的病例系偶然发现的无症状患者,而有症状者在诊断时大都已有或将发生转移病变。目前肾癌的主要治疗方法是外科手术根治,但既使早期患者手术治疗后,仍有部分患者将发生转移。转移性肾癌预后较差,对放化疗等治疗不敏感,3年平均存活率低于5%。因此,深入了解肾癌的发病机制,并在此基础上探索早期诊断和治疗方法有着重要的意义。目的：观察并检测脑组织特异性血管生成抑制因子1(BAI1)基因在癌旁组织、肾透明细胞癌、肾乳头状细胞癌及嫌色细胞癌组织中的表达情况,分析BAI1表达与各临床病理参数之间的相关性,探讨BAI1基因在肾细胞癌发生、发展中的作用,以期为RCC的诊断及治疗提供帮助。方法：1.应用免疫组织化学PV-9001法检测BAI1在60例透明细胞癌、15例乳头状细胞癌、13例嫌色细胞癌和27例正常肾脏组织中的表达情况。2.应用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction; RT-PCR)检测20例癌旁组织、20例肾细胞癌组织中BAI1的mRNA含量。结果：1.BAI1在各种病理类型的RCC中表达比癌旁组织中阳性表达率明显降低,其差异具有统计学意义(P0.05)； 2.BAI1在RCC中的表达与年龄、性别无关(P0.05),但与RCC的Robson分期、病理分级及是否淋巴转移有关(P0.05)。 3.BAI1在肾透明细胞癌中表达与Robson分期呈负相关,其差异具有明显统计学意义(P0.05)。 4. BAIlmRNA在RCC组织中的表达显著低于癌旁组织,其差异具有统计学意义(P0.05)：结论：BAI1基因和mRNA在正常肾癌组织表达阳性率明显高于RCC组织,其表达与肿瘤的分化程度、临床分期、淋巴结转移等临床病理因素存在密切关系,提示BAI1的低表达可能参与RCC的发生发展,并与RCC的侵袭、转移有关。在RCC中检测BAI1基因的表达可能对判断肿瘤的严重程度及诊断治疗提供一定的帮助。
[Abstract]:Renal cell carcinoma (RCC), also called renal cell carcinoma (RCC), accounts for 3% of all malignant tumors in the body, and 75% of its pathological manifestations are clear cell carcinoma. Different pathological types of renal carcinoma have different clinical characteristics, treatment plan and prognosis, hematuria, pain, etc. The mass is called the triple sign of renal cell carcinoma. Clinically, 30 to 50% of the cases are asymptomatic patients found by chance, and most of the patients with symptoms have or will have metastases at the time of diagnosis. At present, the main treatment for renal cell carcinoma is radical surgical treatment. However, even after early surgical treatment, some patients will still have metastasis. The prognosis of metastatic renal cell carcinoma is poor, it is insensitive to radiotherapy and chemotherapy, and the average survival rate of 3 years is lower than 5%. Therefore, we should deeply understand the pathogenesis of renal cell carcinoma. On this basis, it is of great significance to explore the early diagnosis and treatment methods. Objective: to observe and detect the expression of brain tissue specific angiogenesis inhibitor 1 (BAI1) gene in paracancerous tissues, renal clear cell carcinoma, renal papillary cell carcinoma and chromophobe cell carcinoma. To analyze the correlation between the expression of BAI1 and the clinicopathological parameters, and to explore the role of BAI1 gene in the development and development of renal cell carcinoma, in order to provide help for the diagnosis and treatment of RCC. Methods 1. Immunohistochemical PV-9001 method was used to detect the expression of BAI1 in 15 cases of papillary cell carcinoma and 13 cases of chromophobe cell carcinoma and 27 cases of normal kidney tissue. 2. Reverse transcription-polymerase chain reaction (RT-PCR) reverse transcription-polymerase was used to detect the expression of BAI1 in 15 cases of papillary cell carcinoma and 13 cases of chromophobe cell carcinoma and 27 cases of normal renal tissues. Chain reactionation (RT-PCR) was used to detect the mRNA content of BAI1 in 20 cases of renal cell carcinoma (RCC). Results 1. The positive expression rate of BAI1 in RCC was significantly lower than that in paracancerous tissues, and the difference was statistically significant (P 0.05). The expression of 2.BAI1 in RCC was not related to age and sex, but was related to the Robson stage, pathological grade and lymphatic metastasis of RCC. The expression of 3.BAI1 in renal clear cell carcinoma was negatively correlated with Robson stage, and the difference was statistically significant (P 0.05). 4. The expression of BAIlmRNA in RCC was significantly lower than that in paracancerous tissues, and the difference was statistically significant (P 0.05). Conclusion the positive rate of the expression of the mRNA gene in normal renal cell carcinoma tissue is significantly higher than that in the normal renal cell carcinoma tissue. It is closely related to the degree of tumor differentiation, clinical stage, lymph node metastasis and other clinicopathological factors. The results suggest that the low expression of BAI1 may be involved in the occurrence and development of RCC, and may be related to the invasion and metastasis of RCC. The detection of BAI1 gene expression in RCC may be helpful in judging the severity of tumor and in diagnosis and treatment.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.11

【参考文献】