TK1和Ki-67在膀胱尿路上皮癌中的表达及其相关性

发布时间：2018-03-31 14:13

本文选题：膀胱尿路上皮癌　切入点：胸苷激酶1(TK1)　出处：《广西医科大学》2014年硕士论文

【摘要】：目的：探讨胸苷激酶1(thymidine kinase1，TK1)和细胞核增殖相关抗原67(nuclear-associated antigen67，Ki-67)在膀胱尿路上皮癌中的表达及其相关性，了解TK1可否作为膀胱癌的预后因素。方法：收集广西医科大学附属肿瘤医院2010-2013年间病理证实为膀胱尿路上皮癌的病理标本47例和正常膀胱粘膜标本10例。应用免疫组化SP法检测TK1、Ki-67在以上标本中的表达，进而研究TK1和Ki-67的表达与膀胱尿路上皮癌临床病理参数的关系及其相关性。应用SPSS16.0进行统计处理，各组间计数资料的比较采用卡方检验，相关分析采用Spearman分析，以α=0.05为显著性检验水准，P0.05为差异有统计学意义。结果：1. TK1和Ki-67在膀胱尿路上皮癌组织中的表达明显高于正常膀胱黏膜组织，差异有统计学意义（P0.05）。 2.膀胱尿路上皮癌组织中TK1的阳性表达与肿瘤分期有关(P0.05)；Ki-67的阳性表达与肿瘤分期和病理分级均有关（P0.05）。 3.TK1和Ki-67在膀胱尿路上皮癌中的表达成正相关(r=0.371,P=0.001)。结论1.过表达的TK1和Ki-67可能与膀胱黏膜过度增殖和恶性转化有关。 2．在病理分化越差的膀胱尿路上皮癌组织中，，其Ki-67表达水平越高。 3. TK1和Ki-67在与膀胱尿路上皮癌肿瘤分期的关系方面表现出相关性：在肌层浸润性膀胱尿路上皮癌中的表达明显高于非肌层浸润性膀胱尿路上皮癌。 4.本研究用免疫组化方法显示TK1在膀胱尿路上皮癌的表达与膀胱癌的分期密切相关，但在膀胱癌的不同病理分级中表达差异性无显著性统计学意义。TK1是否能作为膀胱癌的预后评估因素，尚需扩大样本及对病人生存期等方面的研究。
[Abstract]:Objective: to investigate the expression of thymidine kinase (1(thymidine kinase1) and nuclear proliferation-associated antigen (67(nuclear-associated antigen67 Ki-67) in bladder urothelial carcinoma, and to investigate whether TK1 can be used as a prognostic factor of bladder cancer.Methods: 47 cases of bladder urothelial carcinoma and 10 cases of normal bladder mucosa were collected from Cancer Hospital affiliated to Guangxi Medical University from 2010 to 2013.Immunohistochemical SP method was used to detect the expression of TK1 Ki-67 in the above specimens, and to study the relationship between the expression of TK1 and Ki-67 and the clinicopathological parameters of bladder urothelial carcinoma.The statistics were processed by SPSS16.0, the data of counting were compared by chi-square test, the correlation analysis by Spearman analysis, using 伪 -0.05as significant test level (P0.05) as the difference was statistically significant.The result is 1: 1.The expression of TK1 and Ki-67 in bladder urothelial carcinoma was significantly higher than that in normal bladder mucosa (P 0.05).2.The positive expression of TK1 in bladder urothelial carcinoma was related to the tumor stage. The positive expression of Ki-67 was correlated with the tumor stage and pathological grade.The expression of 3.TK1 and Ki-67 in bladder urothelial carcinoma was positively correlated with that of 0.371P.Conclusion 1.Overexpression of TK1 and Ki-67 may be associated with excessive proliferation and malignant transformation of bladder mucosa.2.The lower the pathological differentiation, the higher the expression of Ki-67 in bladder urothelial carcinoma.3.The expression of TK1 and Ki-67 in bladder urothelial carcinoma was significantly higher than that in non-myometrial invasive bladder urothelial carcinoma.4.The expression of TK1 in bladder urothelial carcinoma was closely related to the stage of bladder cancer.However, there is no significant difference in the expression of TK1 in different pathological grades of bladder cancer. Whether TK1 can be used as a prognostic factor of bladder cancer, it is necessary to expand the sample and study the survival time of the patients.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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