FcγⅡB I232T基因多态性与膜性肾病易感性的研究

发布时间：2018-04-01 06:41

本文选题：FcγRIB　切入点：基因多态性　出处：《吉林大学》2014年硕士论文

【摘要】：介绍：膜性肾病是成人原发性肾病综合征最常见的病因。其病理显示沿肾小球毛细血管壁四周可发现免疫沉积物包含免疫球蛋白IgG和补体，呈颗粒状分布。Boruchov等的研究表明，Fcγ受体IIB有助于维持B细胞的耐受性，同时提供了第一个论据证明它可能诱导T细胞发挥作用而限制树突状细胞的成熟。因此，这些结果表明Fcγ受体IIB在由免疫复合物介导的疾病发病机制中有重要作用。Fcγ受体IIBI232T是一个功能受损的变异。它已被证实与自身免疫性疾病的易感性相关，如系统性红斑狼疮。但到现在为止，还没有太多的研究来探讨其与免疫复合物介导疾病的相关性，如膜性肾病等。所以在本研究中，我们打算探讨Fcγ受体IIBI232T基因多态性与膜性肾病易感性的关联。材料与方法：80名健康对照组血样和26名膜性肾病患者组血样分别来自于吉林大学中日联谊体检中心和肾内科，标本采集时间为2012年至2013年。膜性肾病的病理诊断由吉林大学基础医学院病理科诊断，组织标本储存于吉林大学中日联谊医院组织标本库。通过实时定量荧光PCR对Fcγ受体IIBI232T进行基因分型。健康组和膜性肾病患者组的I232T基因频率使用标准统计方法进行比较。结果：健康对照组中232I/I型为43例，占53.25%；232I/T型为37例，占46.25%；232T/T型为0例；其中I的基因频率为76.88%，T的基因频率为23.13%。膜性肾病患者组中232I/I型为8例，占30.77%；232I/T型为18例，占69.23%；232T/T型为0例；其中I的基因频率为65.38%，T的基因频率为34.62%。结论：比较FcγRIIBI232T基因多态性在膜性肾病病例组与正常对照组之间的分布，按照基因型方法计算时发现两者存在显著差别，表示FcγRIIBI232T是膜性肾病的危险因素（X2=4.151,P=0.0420.05,OR=2.591）。但按照等位基因呈现度和等位基因频率方法计算时，，两者则无统计学意义（P0.05）。
[Abstract]:This paper introduces that membranous nephropathy is the most common etiology of adult primary nephrotic syndrome. Studies showing granular distribution. Boruchov et al. suggest that FC 纬 receptor IIB contributes to the maintenance of B cell tolerance and provides the first evidence that it may induce T cells to function and limit the maturation of dendritic cells. These results suggest that FC 纬 receptor IIB plays an important role in the pathogenesis of disease mediated by immune complex. FC 纬 receptor IIBI232T is a functionally impaired variant, which has been confirmed to be associated with susceptibility to autoimmune diseases. For example, systemic lupus erythematosus. But so far, there has not been much research on its association with immune complex mediated diseases, such as membranous nephropathy. We intend to explore the association between FC 纬 receptor IIBI232T gene polymorphism and susceptibility to membranous nephropathy. Materials and methods Blood samples of 80 healthy controls and 26 patients with membranous nephropathy were collected from the Sino-Japanese Medical examination Center and the Department of Renal Medicine, Jilin University, respectively. Specimens were collected from 2012 to 2013. The pathological diagnosis of membranous nephropathy was diagnosed by the Department of Pathology, School of basic Medicine, Jilin University. Tissue samples were stored in the tissue library of Sino-Japanese Friendship Hospital of Jilin University. FC 纬 receptor IIBI232T was genotyped by real-time quantitative PCR. The frequency of I232T gene was compared between healthy group and patients with membranous nephropathy. Results: there were 43 cases of 232I/I type, 37 cases of 232I / T type and 0 case of 232T / T type of 46.25 cases in the healthy control group, in which the gene frequency of I was 76.88 T / T 23.13. In the membranous nephropathy group, there were 8 cases of 232I/I type, 18 cases of 30.77232I- / T type and 0 case of 69.23232T / T type. The gene frequency of I was 65.38 and the gene frequency of T was 34.62. Conclusion: the distribution of FC 纬 RIIBI232T gene polymorphism in patients with membranous nephropathy was compared with that in normal controls. The results showed that FC 纬 RIIBI232T was a risk factor for membranous nephropathy.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R692.3

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