慢性肾脏病患者肾组织中Klotho基因启动子羟甲基化水平观察

发布时间：2018-04-03 11:41

本文选题：慢性肾脏病　切入点：Klotho基因　出处：《山东医药》2016年15期

【摘要】：目的观察慢性肾脏病(CKD)患者Klotho基因启动子羟甲基化水平变化,并探讨其与CKD临床病理参数的关系。方法 1~3期CKD患者58例,肾脏穿刺取肾脏组织(CKD组);肾结核患者31例,取手术切除的正常肾脏组织(对照组)。将各组抽提的全基因组DNA分为两份,一份以15 mmol/L高钌酸钾、0.05 mol/L氢氧化钠氧化后,进行亚硫酸盐转化;另一份直接进行亚硫酸盐转化。用特异引物进行PCR扩增,焦磷酸测序,检测羟甲基化并计算其在甲基化中所占比例。分析Klotho基因启动子羟甲基化水平与CKD临床病理参数的关系。结果对照组肾脏组织Klotho基因启动子羟甲基化修饰;CKD组肾脏组织Klotho基因启动子甲基化水平高于对照组,羟甲基化水平低于对照组(P均0.05)。CKD患者Klotho基因启动子羟甲基化水平与e GFR呈正相关关系,与肾小管间质纤维化面积及肌酐水平呈负相关。结论 CKD患者肾脏组织中Klotho基因启动子羟甲基化水平降低,并与CKD临床病理参数有关。Klotho基因启动子羟甲基化水平下降可能参与了CKD的发生发展。
[Abstract]:Objective to observe the level of hydroxymethylation of Klotho gene promoter in patients with chronic kidney disease (CKD) and to explore its relationship with clinicopathological parameters of CKD.Methods (1) 58 patients with stage 3 CKD were treated with renal puncture and 31 patients with renal tuberculosis.The whole genomic DNA extracted from each group was divided into two parts, one was oxidized with sodium hydroxide for 15 mmol/L, and the other was directly transformed by sulfite.Specific primers were used to amplify PCR and pyrosequencing to detect hydroxymethylation and calculate its proportion in methylation.To analyze the relationship between the level of Klotho promoter hydroxymethylation and the clinicopathological parameters of CKD.Results the methylation level of Klotho gene promoter in renal tissue of control group was higher than that of control group.The level of hydroxymethylation of Klotho gene promoter was positively correlated with e GFR, and negatively correlated with the area of renal tubulointerstitial fibrosis and creatinine level.Conclusion the level of hydroxymethylation of the promoter of Klotho gene in the kidney of CKD patients is decreased, and the decrease of the methylation level of the promoter of the Klotho gene may be involved in the pathogenesis and development of CKD, which is related to the clinicopathological parameters of CKD.
【作者单位】：新乡市中心医院;新乡医学院;
【分类号】：R692

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