高糖因素在人肾小球内皮细胞补体凝集素途径激活机制的作用

发布时间：2018-04-04 14:12

本文选题：高糖　切入点：人肾小球内皮细胞　出处：《中国老年学杂志》2016年05期

【摘要】：目的观察高浓度葡萄糖对人肾小球内皮细胞(HRGEC)表面补体凝集素途径(LCP)相关信号通路蛋白表达的影响。方法将HRGEC分3组:高糖组、正常糖组和甘露醇组,分析HRGEC细胞表面甘露聚糖结合凝集素(MBL)、C3及C5b-9沉积的变化。结果 HRGEC表面MBL和C3沉积随糖作用时间的延长而增加,72 h达峰值(P0.05),随糖浓度的升高而增加,60 mmol/L达峰值(P0.05);高糖组HRGEC表面MBL、C3及C5b-9沉积均明显高于正常糖组和甘露醇组(P0.05)。结论葡萄糖可随时间及浓度依赖性促进HRGEC表面MBL和C3沉积;高浓度葡萄糖刺激可能氧化应激诱导HRGEC表面糖基化发生改变,激活LCP途径,引发HRGEC表面C5b-9沉积的增加。
[Abstract]:Aim to investigate the effect of high concentration glucose on the expression of complement lectin pathway (LCP-associated signal pathway) on human glomerular endothelial cells (HRGECs).Methods HRGEC was divided into three groups: high glucose group, normal glucose group and mannitol group. The changes of mannan-binding lectin C _ 3 and C5b-9 on the surface of HRGEC cells were analyzed.Results the deposition of MBL and C3 on the surface of HRGEC increased to a peak value of P0.05A at 72 h and a peak value of P0.05N at 60 mmol/L with the increase of glucose concentration, and the deposition of HRGEC C _ 3 and C5b-9 on the surface of high glucose group was significantly higher than that of normal glucose group and mannitol group.Conclusion glucose can promote the deposition of MBL and C3 on the surface of HRGEC in a time-and concentration-dependent manner, and high glucose stimulation may induce the change of glycosylation on HRGEC surface, activate the LCP pathway, and induce the increase of C5b-9 deposition on HRGEC surface.
【作者单位】：昆明医科大学第一附属医院肾脏内科;昆明医科大学第一附属医院干疗科;四川大学华西医院肾脏内科;
【基金】：2013年度云南省教育厅科学研究基金项目(No.2013Y289)
【分类号】：R692.6

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