香菇多糖与环磷酰胺联合治疗前列腺癌荷瘤小鼠的实验研究

发布时间：2018-04-05 11:38

本文选题：香菇多糖　切入点：环磷酰胺　出处：《中华肿瘤防治杂志》2017年14期

【摘要】：目的前列腺癌具有较高的死亡率,探求其有效治疗方式已成为学者的研究重点。本研究探讨香菇多糖联合环磷酰胺治疗前列腺癌荷瘤小鼠效果及联合治疗机制,为该联合治疗方案临床应用提供依据。方法利用RM-1细胞建立前列腺癌皮下移植小鼠模型,将60只Balb/c荷瘤小鼠随机平分为4组,即对照组(Control组,生理盐水治疗)、LNT组(香菇多糖治疗)、CTX组(环磷酰胺治疗)和LNT+CTX组(香菇多糖和环磷酰胺治疗);观察治疗后荷瘤小鼠肿瘤体积比与平均存活时间,苏木精-伊红(HE)染色观察治疗后肿瘤组织病理学改变,检测荷瘤小鼠脾质量、脾指数和淋巴细胞水平确定荷瘤小鼠免疫功能情况。结果联合治疗后LNT+CTX组荷瘤小鼠肿瘤体积比达到3.51±0.80,显著低于Control组(t=3.174)、LNT组(t=2.730)和CTX组(t=3.086),P0.05;且治疗16d后,LNT+CTX组肿瘤体积比出现下降趋势。HE染色结果进一步表明,LNT组、CTX组和LNT+CTX组肿瘤组织出现明显病理学改变,且LNT+CTX组病理学改变最明显。LNT+CTX组荷瘤小鼠平均存活时间达到60d,显著长于Control组(χ~2=7.747)、LNT组(χ~2=3.653)和CTX组(χ~2=2.784),差异有统计学意义,P0.05。香菇多糖和环磷酰胺联合治疗后,LNT+CTX组荷瘤小鼠脾质量[(0.28±0.08)g]、脾指数[(16.96±2.48)mg/g]及淋巴细胞[CD3+为40.67±3.13,CD4+为25.76±1.90,CD4+/CD8+为1.62±0.24]显著低于LNT组,但高于CTX组和Control组,均P0.05。治疗后各组荷瘤小鼠CD8+水平差异无统计学意义,P0.05。结论香菇多糖与环磷酰胺联合作用共同减缓了肿瘤生长,提高了荷瘤小鼠存活时间,其可能机制是香菇多糖提高了环磷酰胺治疗小鼠的免疫功能,促进了对肿瘤杀伤。
[Abstract]:Objective Prostate cancer has a high mortality rate, and it has become the focus of scholars to explore its effective treatment.The purpose of this study was to investigate the therapeutic effect of lentinan combined with cyclophosphamide on prostate cancer bearing mice and to provide evidence for clinical application of the combined therapy.Methods RM-1 cells were used to establish subcutaneous transplantation model of prostate cancer in mice. 60 Balb/c mice were randomly divided into 4 groups: control group (control group), control group (control group), and control group (control group).The tumor volume ratio and mean survival time of tumor-bearing mice were observed after treatment with Lentinus edodes (Lentinus edodes) group (CTX group) and LNT CTX group (Lentinus edodes polysaccharides group and cyclophosphamide group).Hematoxylin-eosin (HEH) staining was used to observe the pathological changes of tumor tissues, and to determine the immune function of tumor-bearing mice by detecting the spleen weight, spleen index and lymphocyte level of tumor-bearing mice.There were obvious pathological changes in tumor tissue in CTX group.The average survival time of the tumor-bearing mice in the LNT CTX group was 60 days, which was significantly longer than that in the Control group (蠂 2 + 7.747 L NT group) and the CTX group (蠂 2 + 2.784) group (P 0.05).After treatment, there was no significant difference in CD8 level in tumor bearing mice in each group (P 0.05).Conclusion the combined action of lentinan and cyclophosphamide can slow down the growth of tumor and increase the survival time of tumor-bearing mice. The possible mechanism is that Lentinus edodes polysaccharides can improve the immune function of mice treated with cyclophosphamide and promote the killing of tumor.
【作者单位】：新疆维吾尔自治区中医院外二科;
【分类号】：R737.25

【相似文献】