敲减BLM解旋酶表达增强前列腺癌PC3细胞对丝裂霉素C的敏感性

发布时间：2018-04-05 16:06

本文选题：丝裂霉素C　切入点：前列腺癌细胞　出处：《中国生物化学与分子生物学报》2017年02期

【摘要】：丝裂霉素C是一种广谱抗肿瘤抗生素,对多种癌症有抗癌作用,其作用原理可使细胞的DNA发生链间交联,引起DNA双链断裂,阻碍DNA的复制,从而抑制肿瘤细胞分裂。临床上主要用于胃癌、肠癌、肝癌及胰腺癌等消化道癌方面的治疗。本文研究丝裂霉素C对转染人BLM解旋酶基因(shRNA载体)前后前列腺癌PC3细胞活性的影响。使用前期成功构建的干扰载体转染PC3细胞,在转染48 h后加药,通过荧光定量PCR、MTT法、Transwell小室实验、细胞划痕实验、流式细胞术,分别检测加药12、24、36 h BLM基因的表达量、PC3细胞增殖能力、侵袭能力、迁移能力及凋亡情况的变化。结果显示,敲减BLM基因表达后的PC3细胞相对于正常PC3细胞其增殖能力、侵袭能力和迁移能力能显著被丝裂霉素C抑制,且丝裂霉素C能显著促进其细胞的凋亡,说明BLM基因低表达的前列腺癌细胞对丝裂霉素C更敏感。研究结果为丝裂霉素C在前列腺癌的临床治疗上奠定了理论基础。
[Abstract]:Mitomycin C is a broad-spectrum antitumor antibiotic, which has anticancer effect on many kinds of cancer. Its mechanism can cause interstrand crosslinking of DNA in cells, cause double strand break of DNA, block the replication of DNA, and thus inhibit the division of tumor cells.It is mainly used in the treatment of gastrointestinal cancer, such as stomach cancer, intestinal cancer, liver cancer and pancreatic cancer.The effect of mitomycin C on the activity of prostate cancer PC3 cells before and after transfection of human BLM helicase gene was studied.After 48 hours of transfection, PC3 cells were transfected with interference vector successfully constructed before transfection.The expression of BLM gene and the changes of proliferation, invasion, migration and apoptosis of PC3 cells were detected.The results showed that the ability of proliferation, invasion and migration of PC3 cells after knockout of BLM gene expression was significantly inhibited by mitomycin C, and mitomycin C could significantly promote the apoptosis of PC3 cells.It suggested that prostate cancer cells with low expression of BLM gene were more sensitive to mitomycin C.The results provide a theoretical basis for the clinical treatment of prostate cancer with mitomycin C.
【作者单位】：贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州大学生命科学学院;贵州大学动物科学学院;
【基金】：国家自然科学基金(No.31361406)资助~~
【分类号】：R737.25

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