苯那普利对糖尿病肾病大鼠肾脏结缔组织生长因子表达的调节作用

发布时间：2018-04-06 07:06

本文选题：肾组织　切入点：苯那普利　出处：《中国老年学杂志》2017年15期

【摘要】：目的研究苯那普利对糖尿病肾病(DN)大鼠肾脏结缔组织生长因子(CTGF)表达的调节。方法 Wistar大鼠34只,随机分为:对照组、DN、苯那普利组。DN组及苯那普利组大鼠经腹腔注射链脲佐菌素(STZ)建立DN模型。于实验第12周末处死大鼠测量大鼠体质量、空腹血糖、肾功能、尿白蛋白排泄率(UAER)及24 h尿总蛋白。应用免疫组化方法检测肾脏CTGF蛋白表达,并进行半定量分析。结果与对照组相比,DN组大鼠空腹血糖、血尿素氮、血清肌酐、UAER及24h尿总蛋白显著升高(P0.05或P0.01);肾脏CTGF表达增加(P0.05)。DN大鼠苯那普利组各项检测指标均较模型组显著改善(P0.05或P0.01)。结论苯那普利可下调DN苯那普利肾组织CTGF的表达,降低蛋白尿,保护肾功能。
[Abstract]:Objective to study the effect of benazepril on the expression of connective tissue growth factor (CTGF) in kidney of diabetic nephropathy rats.Methods 34 Wistar rats were randomly divided into control group, benazepril group. DN group and benazepril group were injected intraperitoneally with streptozotocin (STZ) to establish DN model.At the end of the 12th week, the rats were killed to measure the body mass, fasting blood glucose, renal function, urinary albumin excretion rate (UAER) and 24 h urinary total protein.The expression of CTGF protein in kidney was detected by immunohistochemistry and semi-quantitative analysis was carried out.Results compared with the control group, fasting blood glucose, blood urea nitrogen, serum creatinine UAER and 24 h urinary total protein were significantly increased in DN group (P0.05 or P0.01), and the expression of renal CTGF in benazepril group was significantly improved compared with model group (P0.05 or P0.01).Conclusion benazepril can down-regulate the expression of CTGF in renal tissue of DN benazepril, decrease proteinuria and protect renal function.
【作者单位】：北华大学基础医学院病理教研室;北华大学基础医学院生理教研室;北华大学附属医院病理科;吉林大学再生医学科学研究所;
【分类号】：R587.2;R692.9

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