人膀胱癌脱落细胞捕获及其基因检测分析

发布时间：2018-04-06 23:35

本文选题：膀胱癌　切入点：脱落　出处：《北京化工大学》2014年硕士论文

【摘要】：膀胱癌是人泌尿系统中一种常见的癌症,非侵入性早期检测膀胱癌在临床诊断上具有重要的意义,并且得到了广泛的研究。本文通过研究尿液中脱落细胞捕获和研究肿瘤相关基因检测两方面内容,提高了细胞的捕获效率,增强了检测的灵敏度,希望应用于膀胱癌的早期临床诊断或者预后中。研究的主要内容和结论如下：第一章综述了体液中脱落肿瘤细胞的分离捕获方法,详细阐述了膀胱癌早期检测的方法及最新进展。在此基础上提出了论文的研究思路和方案。第二章膀胱癌发生发展过程中肿瘤细胞会脱落到尿液中,为非侵入性检测膀胱癌提供了可能。本文通过采用聚碳酸酯微孔膜和镍微孔筛快速高效的捕获尿液中的膀胱癌细胞,并且利用二氯甲烷溶膜的特性,简单快捷的回收捕获在聚碳酸酯微孔滤膜上的细胞。微孔过滤法捕获细胞效率85%,分离富集时间20min,明显优于临床上传统的离心分离富集方法。本实验较为系统的考察了滤膜类型、固定剂以及流速对肿瘤细胞捕获效率的影响,该方法速度快,成本低,并且环境友好,为今后应用于临床诊断研究提供了可能性及理论依据。第三章体细胞突变和甲基化在肿瘤发生过程中起着重要作用。本文设计了通过基因分析非侵入性检测膀胱癌的标志物,该方法包括体细胞突
[Abstract]:Bladder cancer is a common cancer in human urinary system. Noninvasive early detection of bladder cancer is of great significance in clinical diagnosis and has been widely studied.In this paper, we studied the capture of exfoliated cells in urine and the detection of tumor-related genes in order to improve the efficiency of cell capture and enhance the sensitivity of detection. We hope to be used in the early clinical diagnosis or prognosis of bladder cancer.The main contents and conclusions of the study are as follows:In the first chapter, the methods of isolation and capture of tumor cells from body fluid are reviewed, and the methods and recent progress of early detection of bladder cancer are described in detail.On the basis of this, the paper's research ideas and schemes are put forward.In Chapter 2, the tumor cells fall off into urine during the development of bladder cancer, which provides the possibility for noninvasive detection of bladder cancer.In this paper, polycarbonate microporous membrane and nickel microporous screen were used to capture bladder cancer cells in urine rapidly and efficiently, and the characteristics of dichloromethane membrane were used to recover the cells captured on polycarbonate microporous filter membrane.The micropore filtration method was superior to the traditional centrifugation and enrichment method in capturing cell efficiency and separating and enriching cells for 20 min.The effects of filter membrane type, fixation agent and flow rate on the capture efficiency of tumor cells were investigated systematically in this experiment. The method was fast, low cost and environmentally friendly.It provides the possibility and theoretical basis for clinical diagnosis in the future.Chapter 3 somatic mutation and methylation play an important role in tumorigenesis.A noninvasive marker for the detection of bladder cancer by gene analysis has been designed. This method includes somatic processes.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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