ssp411基因敲除小鼠生育表型的初步研究

发布时间：2018-04-12 08:28

本文选题：转基因动物模型 + ssp　；参考：《中华生殖与避孕杂志》2017年09期

【摘要】：目的对ssp411基因的生育功能进行初步研究。方法利用PB转座子插入建立小鼠模型ssp411基因敲除小鼠各基因型之间交配后,检查雌鼠生殖道是否见栓。记录出生小鼠的窝仔数,性别,出生20 d仔鼠体质量、基因型等数据;记录出生后90 d雄仔鼠的睾丸重量,进行组织形态学研究,并对附睾尾精子进行活力分析。结果 ssp411缺陷型雌鼠可以正常生育,而雄鼠无自然生育能力。ssp411基因敲除雄鼠能够与母鼠自然交配形成阴道栓,但是没有子代出生,且在交配后母鼠输卵管壶腹部未见精子。睾丸的组织形态学研究和睾丸称量结果表明,ssp411蛋白缺失可以影响精子的生成。ssp411基因敲除雄鼠的附睾精子活力也明显差于对照组。结论 ssp411基因缺陷不影响雌性生殖,但影响雄鼠的精子生成功能,造成少弱精子症,并引起雄性不育。
[Abstract]:A preliminary study on the reproductive function of objective ssp411 gene. Methods using PB transposon insertion to establish mouse model of ssp411 gene knockout mice after mating between each gene type, check whether the female reproductive tract. See the bolt number, litter birth records of mice sex, birth 20 d rats body weight, genotype data; records of 90 d male rats testis weight after birth, tissue morphology, and epididymal sperm vitality were analyzed. Results ssp411 deficient female rats and male rats to normal fertility, no natural fertility of.Ssp411 knockout male mice and rats to natural mating formation of the suppository. But no offspring born. In the mating and stepmother rat ampulla of fallopian tube sperm. No testicular tissue morphology of the testis and the weighing result showed that ssp411 protein deficiency can affect the generation of sperm.Ssp411 gene knockout male mice sperm Conclusion the ssp411 gene defect does not affect female reproduction, but affects the Spermatogenesis Function of male mice, resulting in oligospermia and spermatogenesis, and causes male infertility.

【作者单位】：上海市计划生育科学研究所国家人口和计划生育避孕药具重点实验室;复旦大学生命科学学院;
【基金】：国家自然科学基金(81471503)~~
【分类号】：R698.2

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