组胺与5-羟色胺对膀胱过度活动症大鼠膀胱功能的影响

发布时间：2018-04-15 16:40

本文选题：过度活动性膀胱 + 下尿路症状　；参考：《第二军医大学学报》2017年01期

【摘要】：目的探索低剂量的组胺与5-羟色胺对膀胱过度活动症(OAB)大鼠膀胱功能的影响。方法对40只SD雌性大鼠实施膀胱出口不全梗阻手术(PBOO),模拟OAB的临床尿动力学改变,术后将大鼠随机分为4组:2μg组胺(10μg/mL)治疗组、1.1μg 5-羟色胺(5.5μg/mL)治疗组、2μg组胺与1.1μg 5-羟色胺联合用药组及模型对照组。另外10只SD雌性大鼠予以假手术并用生理盐水处理。药物及生理盐水治疗均开始于术后第10天,给药方法为皮下注射,每日2次,持续1周。通过清醒状态下的膀胱压力测定记录各组大鼠储尿期与排尿期的尿动力学指标,完毕后收取膀胱组织称量并进行组织学分析。采用单因素方差分析和Tukey事后多重比较方法对组间的定量数据进行统计学分析。结果与假手术组相比,模型对照组、组胺治疗组、5-羟色胺治疗组及组胺与5-羟色胺联合用药组大鼠的逼尿肌不稳定收缩的频率、振幅、膀胱最大容量、排尿收缩的间隔时间、残余尿量以及膀胱质量均有提升(P0.05)。与模型对照组相比较,仅联合用药组大鼠的排尿收缩振幅有提升,不稳定收缩的振幅与残余尿量均降低(P0.05)。组织学分析表明联合用药与单用组胺治疗组大鼠逼尿肌纤维连续且排列紧密。结论联合应用低剂量的组胺与5-羟色胺可有效改善OAB大鼠储尿期膀胱功能的稳定性,并提高膀胱的排空效率,可为临床OAB治疗提供一种新的思路。
[Abstract]:Objective to investigate the effects of low dose histamine and 5-hydroxytryptamine on bladder function in rats with bladder hyperactivity.Methods 40 female Sprague-Dawley rats were operated on bladder outlet incomplete obstruction and the clinical urodynamic changes of OAB were simulated.The rats were randomly divided into 4 groups: 1. 1 渭 g 5 渭 g histamine and 1. 1 渭 g hydroxytryptamine combined with 1. 1 渭 g histamine and 1. 1 渭 g serotonin, and model control group.Another 10 SD female rats were sham-operated and treated with normal saline.Both drugs and saline were given subcutaneously twice a day for 1 week.The urinary dynamic indexes of rats in the period of urine storage and urination were recorded by the measurement of bladder pressure in awake state, then the weight of bladder tissue was collected and the histological analysis was carried out.Single factor analysis of variance (ANOVA) and Tukey post-multiple comparison were used to analyze the quantitative data between groups.Results compared with sham operation group, model control group, histamine treatment group and histamine combined with serotonin group, the detrusor unsteady contraction frequency, amplitude, maximum bladder volume and interval time of urination contraction were observed.The residual urine volume and bladder mass were all increased (P 0.05).Compared with the model control group, the contraction amplitude of micturition was increased, the amplitude of unstable contraction and the residual urine volume were decreased in the combined treatment group (P 0.05).Histologic analysis showed that the detrusor fibers of the combined treatment group and the single histamine group were continuous and closely arranged.Conclusion the combination of low dose histamine and 5-hydroxytryptamine can effectively improve the stability of urinary bladder function in OAB rats and improve the bladder emptying efficiency. It can provide a new idea for clinical treatment of OAB.
【作者单位】：第二军医大学长海医院泌尿外科;
【基金】：国家自然科学基金青年科学基金(81500579)~~
【分类号】：R694.5

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