酒对高脂饮食家兔肾脏的影响

发布时间：2018-04-16 12:24

本文选题：高脂血症 + 饮酒　；参考：《桂林医学院》2014年硕士论文

【摘要】：目的肥胖、高脂血症、心脑血管疾病等已严重威胁人类健康，本研究通过对家兔灌酒及高脂喂饲来制备家兔的高脂血症模型，用以揭示不同白酒灌服量对高脂饮食新西兰兔的血脂、血糖及肾功能的影响。方法将36只新西兰兔随机分为6组，每组6只。A组：饲以普通颗粒饲料；B组：饲以高脂饲料（配方：1%胆固醇，7.5%蛋黄粉，3%猪油，88.5%基础饲料）；C组：饲以普通颗粒饲料+白酒1.5g/kg·d；D组：予高脂喂养及白酒0.3g/kg·d；E组：予高脂喂养及白酒0.6g/kg·d；F组：予高脂喂养及白酒1.5g/kg·d。每兔每日进食量为120g，酒量均分2次灌服，2次间隔8h，单笼喂养、饮水不限。各组家兔维持饮食和灌酒连续12周。分别于实验开始前（0周）、第4、8、12周末留取全血、尿标本，观察家兔血清中葡萄糖(GLU)、总胆固醇(TCH)、甘油三酯(TG)、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、尿素氮（BUN）、肌酐（Cr）和尿β2微球蛋白（β2MG）的含量变化。于12周末处死新西兰兔取肾，通过HE、Masson染色观察判断肾小球和肾小管的病理变化。结果1)血脂、血糖水平：不同浓度饮酒均能降低高脂饮食家兔血清甘油三酯TG；低浓度饮酒能轻度降低低密度脂蛋白LDL（P＜0.05）；中浓度饮酒第4周、第8周低密度脂蛋白LDL升高，第12周低密度脂蛋白LDL下降（P＜0.05）；高浓度饮酒的低密度脂蛋白LDL明显升高，高密度脂蛋白HDL早期升高后期降低（P＜0.05）；大量饮酒和高脂饮食均显著升高血糖（P＜0.01）。2)肾功能：单纯高脂饮食和单纯饮酒家兔尿β2微球蛋白均升高（P＜0.05），BUN和Cr未见显著变化；高脂饮食+不同浓度饮酒使BUN、Cr和β2MG均升高（P＜0.05），，且升高幅度与饮酒浓度和时间成正相关（P＜0.05）。3)肾组织病理检查：各组肾小球硬化系数比较，C组与A组差别无统计学意义（P＞0.05），B、D、E、F组均显著高于A组（P＜0.05或P＜0.01）；与B组相比较，C组和D组无统计学意义（P＞0.05），E组和F组明显高于B组（P＜0.05或P＜0.01）；与C组比较，D、E、F组显著增高（P＜0.05或P＜0.01）。各组肾小管损伤分数比较，B、C、D、E、F组均显著高于A组（P＜0.01），与B组相比较， B、C、D、E、F组均明显增高（P＜0.01）；与C组比较，D、E、F组显著增高（P＜0.05或P＜0.01）。结论适度饮酒能降低高脂饮食所致高脂血症，大量饮酒和高脂饮食导致脂质代谢紊乱、血糖升高及肾功能损伤。长时间单纯饮酒与单纯高脂饮食也会导致肾脏组织损伤，在高脂饮食基础上的不同程度饮酒会加重肾脏组织损害，而且随饮酒量增加其肾脏损伤严重程度明显增加，可导致严重的肾间质纤维化和肾小球硬化
[Abstract]:Objective Obesity, hyperlipidemia, cardiovascular and cerebrovascular diseases have seriously threatened human health. In this study, the hyperlipidemia model of rabbits was established by drinking and feeding hyperlipidemia.The effects of different doses of liquor on blood lipids, blood glucose and renal function of New Zealand rabbits with high fat diet were studied.Methods 36 New Zealand rabbits were randomly divided into 6 groups.Group A: normal pellet feed group B: fed with high fat diet (1% 1% cholesterol, 7.5% egg yolk powder, 3% lard), group C: 1.5g/kg D group: high fat diet and white wine 0.3g/kg dai group: high fat feeding and liquor 0.6g/kg d F group: high fat feeding and liquor 1.5g/kg d.The amount of food consumed was 120 g per day, and the average amount of alcohol was divided into 2 times, 2 times interval 8 hours, single cage feeding, drinking water unlimited.The rabbits in each group were fed and perfused for 12 weeks.The whole blood and urine samples were collected at the end of the 4th week and the 12th week before the beginning of the experiment.The changes of serum glucose, TCHG, TGG, LDLX, HDLX, bun, Cr and 尾 2 microglobulin (尾 2MGG) in serum of rabbits were observed.At the end of 12 th week, New Zealand rabbits were sacrificed and the pathological changes of glomeruli and renal tubules were observed by HE Masson staining.Results 1) Serum lipids and blood glucose levels: different concentrations of alcohol drinking could decrease serum triglyceride level in rabbits with high fat diet, low concentration alcohol drinking could slightly reduce LDL(P of LDL < 0.05, moderate concentration of alcohol drinking could increase the level of LDL LDL at the 4th week and the 8th week after drinking.Low density lipoprotein (LDL) decreased at the 12th week (P < 0.05), and the low density lipoprotein (LDL) increased significantly in high alcohol drinking.High density lipoprotein (HDL) HDL decreased P < 0.05 in the early stage and increased significantly in both heavy drinking and high-fat diet (P < 0.01). Renal function was significantly increased in both high fat diet and drinking alone. No significant changes were found in urinary 尾 2-microglobulin (P < 0.05 bun and Cr) in rabbits with high fat diet and drinking alone.Drinking alcohol at different concentrations of high-fat diet increased bun Cr and 尾 2MG (P < 0.05), and the amplitude of increase was positively correlated with drinking concentration and time (P < 0.05) pathological examination of renal tissue: there was no significant difference in glomerulosclerosis coefficient between group C and group A (P > 0.05).There was no significant difference between group C and group D (P > 0.05) and group F (P < 0.05 or P < 0.01), and compared with group C (P < 0.05) and group D (P < 0.01), group D was significantly higher than group C (P < 0.05 or P < 0.01).The scores of renal tubule injury in group B were significantly higher than those in group A (P < 0.01), and those in group B were significantly higher than those in group B (P < 0.05 or P < 0.01) and in group C (P < 0.05 or P < 0.01).Conclusion moderate drinking can reduce hyperlipidemia induced by high fat diet, heavy drinking and high fat diet lead to disorder of lipid metabolism, increase of blood glucose and damage of renal function.Drinking alcohol for a long time and high fat diet for a long time would also lead to renal tissue injury, and different degrees of drinking on the basis of high fat diet would aggravate the renal tissue damage, and the severity of renal injury would increase with the increase of alcohol consumption.Can lead to severe interstitial fibrosis and glomerulosclerosis
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R589.2;R692

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