比格犬阴茎移植模型建立的初步探讨

发布时间：2018-04-23 08:55

本文选题：阴茎 + 解剖　；参考：《中华男科学杂志》2017年08期

【摘要】：目的:探讨成年比格犬作为同种异体阴茎移植动物模型可行性,进一步探讨稳定的阴茎移植动物模型建立条件。方法:按照实验动物模型建立原则,进行移植阴茎相似性、重复性、可行性、适用性和可控性研究。20只成年比格犬阴茎与10例成人阴茎解剖学要点比较,分析主要存在的共同特点和相似性;10对成年比格犬阴茎在显微外科技术辅助下进行交叉移植,并采用FK506+MMF+MP免疫抑制方案,观察移植阴茎存活情况;10只显微外科技术辅助比格犬阴茎离断再植术相关指标进行可操作性比较。结果:20只成年比格犬阴茎解剖学观察与10例成人阴茎解剖学观察具有高度相似性,相互比较统计学上无明显差异。10对成年比格犬阴茎交叉移植手术均获得成功,术后8只出现移植阴茎坏死,12只移植阴茎成功存活,恢复排尿功能;显微外科技术辅助下一次性静脉吻合成功率达95.0%(38/40),一次性动脉吻合成功率达87.5%(35/40),血管吻合时间(71.0±9.0)min,手术时间(133.0±10.3)min,失血量(135.8±41.4)ml。比较10只显微外科技术辅助下比格犬阴茎离断再植手术,一次性静脉吻合成功率达100%(20/20),一次性动脉吻合成功率达90.0%(18/20),血管吻合时间(65.0±7.9)min,手术时间(117.4±10.0)min,失血量(85.0±10.8)ml。12例临床显微外科技术辅助下成人阴茎离断再植手术,一次性静脉吻合成功率达100%(24/24),一次性动脉吻合成功率达95.8%(23/24),血管吻合时间(79.0±17.6)min,手术时间(125.0±20.6)min,失血量(140.0±44.3)ml。3组资料经统计学分析显示各项指标无显著性差异。结论:比格犬阴茎海绵体结构与人阴茎海绵体结构具有高度相似性,在阴茎海绵体横断面解剖中其主要解剖结构均相同;显微外科技术辅助下行比格犬阴茎再植和同种异体移植手术均获得了成功,其手术操作过程中各项指标与临床中人阴茎再植手术相比较,具有相近的可操作性、重复性和较强的可行性。因此,采用成年比格犬作为实验动物建立阴茎移植手术模型切实可行。
[Abstract]:Objective: to investigate the feasibility of adult Beagle as an animal model of penis allograft and to explore the conditions for establishing a stable penis transplantation model. Methods: according to the principle of establishing experimental animal model, the similarity, repeatability, feasibility, applicability and controllability of penis transplantation were studied. 20 adult Beagle penises were compared with 10 adult penis anatomical points. To analyze the common characteristics and similarities of the common features and similarities, we performed cross transplantation of adult Beagle penis with the aid of microsurgical technique, and adopted FK506 MMF MP immunosuppressive protocol. To observe the survival of the transplanted penis and to compare the relevant indexes of 10 microsurgical assisted Biger dogs with replantation of the penis. Results the penile anatomy of 20 adult Beagle dogs was highly similar to that of 10 adults. After operation, 8 cases had necrotic penis grafts and 12 of them survived successfully, and recovered urination function. The success rate of one-off venous anastomosis was 95.0 / 40, and the successful rate of one-off arterial anastomosis was 87.5a / 35 / 40m, the time of vascular anastomosis was 71.0 卤9.0min, the operative time was 133.0 卤10.3min, and the blood loss was 135.8 卤41.4ml / ml. To compare the microsurgical technique in 10 Beagle dogs with penile amputation and replantation. The success rate of one-off venous anastomosis was 100% 20 / 20, and the success rate of one-off arterial anastomosis was 90.00.18 / 20, the time of anastomosis was 65.0 卤7.9 min, the operative time was 117.4 卤10.0 min, and the amount of blood loss was 85.0 卤10.8)ml.12 assisted by clinical microsurgical technique. The success rate of one-off venous anastomosis was 100% 24 / 24, the success rate of one-off arterial anastomosis was 95.880 / 24, the time of vascular anastomosis was 79.0 卤17.6 min, the operative time was 125.0 卤20.6 min, and the blood loss was 140.0 卤44.3)ml.3. There was no significant difference between the two groups by statistical analysis. Conclusion: the structure of the corpus cavernosum of the Beagle dog is highly similar to that of the human corpus cavernosum, and its main anatomical structure is the same in the cross-sectional anatomy of the corpus cavernosum of the penis. The microsurgical technique was used to assist in the operation of penis replantation and allograft transplantation in Beagle dogs. The indexes of the operation were similar to those of the clinical human penis replantation and had similar maneuverability. Repeatability and strong feasibility. Therefore, it is feasible to establish the model of penis transplantation by using adult Beagle as experimental animal.
【作者单位】：广州军区广州总医院泌尿外科;
【基金】：国家自然科学基金(30872577)~~
【分类号】：R-332;R699.8

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