双歧杆菌介导CTP-NPRL2对裸鼠肾癌生长及凋亡的影响

发布时间：2018-04-25 13:11

  本文选题：肾肿瘤 + 双歧杆菌　； 参考：《重庆医学》2017年18期

【摘要】：目的探讨双歧杆菌介导CTP-NPRL2对裸鼠肾癌生长及凋亡的影响。方法构建pET15b-CTP-NPRL2并电转双歧杆菌,Western blot验证蛋白表达。采用肾癌细胞悬液皮下注射法建立裸鼠肾癌模型,并分为观察组与对照组(每组8只),分别经尾静脉注射携带pET15b-CTP-NPRL2的双歧杆菌与生理盐水,每周1次,4周后处死裸鼠,称取裸鼠及荷瘤质量,TUNEL法检测肾癌组织凋亡。结果观察组与对照组裸鼠质量分别为(26.24±1.98)g、(23.28±2.17)g,荷瘤质量分别为(1.37±0.12)g、(1.68±0.18)g,两组比较差异均有统计学意义(P0.05)。TUNEL检测显示观察组凋亡指数(23.27±5.14)%明显高于对照组(10.37±2.58)%,两组比较差异有统计学意义(P0.05)。结论双歧杆菌介导CTP-NPRL2对裸鼠肾癌生长具有抑制功能,并明显增加了肾癌细胞的凋亡。
[Abstract]:Objective to explore the effect of bifidobacteria mediated CTP-NPRL2 on the growth and apoptosis of nude mice kidney cancer. Methods to construct pET15b-CTP-NPRL2 and convert Bifidobacterium and Western blot to verify protein expression. The renal carcinoma model of nude mice was established by subcutaneous injection of renal cell suspension, and divided into the observation group and the control group (8 rats in each group), and the pE was injected into the tail vein to carry pE respectively. T15b-CTP-NPRL2 of Bifidobacterium and saline, 1 times a week, 4 weeks after the death of nude mice, called nude mice and tumor bearing mass, TUNEL method to detect the apoptosis of renal carcinoma tissue, the results of the observation group and the control group were (26.24 + 1.98) g, (23.28 + 2.17) g, the mass of the tumor was (1.37 + 0.12) g, (1.68 + 0.18) g, and the difference of the two groups was statistically significant P0.05.TUNEL detection showed that the apoptotic index (23.27 + 5.14)% of the observation group was significantly higher than that of the control group (10.37 + 2.58)%, and the two groups had a significant difference (P0.05). Conclusion Bifidobacterium mediated CTP-NPRL2 inhibited the growth of nude mice and increased the apoptosis of renal cell carcinoma cells.

【作者单位】： 重庆市永荣矿业有限公司总医院泌尿外科;重庆医科大学附属第一医院泌尿外科;
【基金】：重庆市卫生局面上项目(2013-2-262)
【分类号】：R737.11
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本文编号：1801471