坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响

发布时间：2018-04-25 14:13

本文选题：坦索罗辛 + 前列腺癌PC-细胞　；参考：《中国药房》2016年07期

【摘要】：目的:研究坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响。方法:以0(空白对照组)、12.5、25、50μmol/L坦索罗辛(坦索罗辛低、中、高浓度组)作用于PC-3细胞48 h后,通过MTT法检测细胞活力,Hoechst 33258染色检测细胞凋亡率,Western blot法检测核糖体Bax、B淋巴细胞瘤2(Bcl-2)蛋白表达及蛋白激酶B(Akt)、哺乳动物雷帕霉素靶蛋白(m TOR)、S6蛋白激酶(p70S6K)、4E结合蛋白1(4E-BP1)的磷酸化水平。结果:与空白对照组比较,坦索罗辛低、中、高浓度组PC-3细胞活力和Akt、p70S6K、4E-BP1磷酸化水平均降低,Bax蛋白表达水平升高(P0.05或P0.01);坦索罗辛中、高浓度组PC-3细胞的凋亡率升高,Bcl-2蛋白表达和m TOR磷酸化水平降低(P0.01),上述效果均呈浓度依赖性。结论:坦索罗辛能抑制前列腺癌PC-3细胞增殖并诱导其凋亡,其机制可能与抑制Akt/m TOR信号通路激活有关。
[Abstract]:Aim: to study the effects of tamsoloxine on the proliferation and apoptosis of prostate cancer PC-3 cells. Methods: PC-3 cells were treated with 0 (normal control group, 12.5U 2550 渭 mol/L) with tansoloxine (low, medium and high concentrations) for 48 h. The expression of ribosomal Baxon B lymphocytoma 2 (Bcl-2) protein and the phosphorylation level of protein kinase BX Akt1 and mammalian rapamycin target protein protein kinase p70S6 protein kinase p70S6KO4E binding protein were detected by MTT assay and Western blot assay. Results: compared with the control group, the activity of PC-3 cells and the phosphorylation level of Aktp70S6Kn4E-BP1 in the high concentration group were lower than those in the blank control group. The apoptotic rate of PC-3 cells in high concentration group increased the expression of Bcl-2 protein and the phosphorylation level of m TOR decreased P0.01. the above effects were both concentration-dependent. Conclusion: tamsoloxine can inhibit the proliferation and induce apoptosis of prostate cancer PC-3 cells, and its mechanism may be related to the inhibition of activation of Akt/m TOR signaling pathway.
【作者单位】：天津中医药大学第一附属医院泌尿外科;
【分类号】：R737.25

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