乌梅提取物对纳米细菌致大鼠肾损伤的修复与拮抗结石生成的关系研究

发布时间：2018-04-27 06:01

本文选题：乌梅提取物 + 纳米细菌　；参考：《中国医科大学学报》2017年07期

【摘要】：目的构建纳米细菌(NB)致肾结石大鼠模型,观察乌梅提取物对早期肾损伤分子1(KIM-1)和骨桥蛋白(OPN)的影响,分析乌梅提取物对早期肾结石的治疗意义。方法从人上尿路结石分离并培养NB。将54只雄性SD大鼠随机分为空白组、诱石组和治疗组,采取尾静脉注射NB构建肾结石模型,以乌梅提取物干预模型,并在不同时期分批处死大鼠,实时PCR检测肾组织KIM-1 m RNA的表达,ELISA检测尿KIM-1浓度,HE染色观察肾组织结石晶体情况,并用免疫组化方法检测OPN表达。结果诱石组及治疗组肾结石模型大鼠出现肾小管扩张,肾小管结石晶体形成,早期KIM-1和OPN的表达升高,上述变化与NB的注射时间呈正相关,乌梅提取物可拮抗该类变化。结论乌梅提取物可能通过修复肾损伤减少肾结石形成,该机制可能与乌梅提取物对KIM-1、OPN的基因调节有关。
[Abstract]:Objective to establish a rat model of renal calculi induced by nanometer bacteria (NB) and to observe the effects of the extract of black plum on the early renal injury molecule (1KIM-1) and osteopontin (OPN), and to analyze the therapeutic significance of the extract on early renal calculi. Methods NBs were isolated and cultured from upper urinary tract stones. Fifty-four male Sprague-Dawley rats were randomly divided into three groups: the blank group, the induced stone group and the treatment group. The kidney stone model was established by injecting NB into the tail vein. The expression of KIM-1 m RNA in renal tissue was detected by real-time PCR and the expression of OPN in renal tissue was detected by Elisa and HE staining of urinary KIM-1 concentration. Results Renal tubule dilatation, renal tubulithiasis crystal formation and the expression of KIM-1 and OPN in early stage were found in the model rats of induced stone group and treatment group. These changes were positively correlated with the injection time of NB, which could be antagonized by the extract of Wumei. Conclusion the extract may reduce the formation of renal calculi by repairing renal injury, and this mechanism may be related to the gene regulation of KIM-1 OPN.
【作者单位】：贵州医科大学附属医院泌尿外科;
【基金】：贵阳市科技计划(2014100145)
【分类号】：R692.4

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