基质Gla蛋白与骨形成蛋白2在草酸钙肾结石患者Randall钙斑中的表达

发布时间：2018-04-29 09:37

本文选题：基质Gla蛋白 + 骨形成蛋白　；参考：《中南大学学报(医学版)》2017年03期

【摘要】：目的:对比基质Gla蛋白(matrix Gla protein,MGP)及骨形成蛋白2(bone morphogenetic protein 2,BMP-2)在草酸钙结石肾乳头Randall钙化斑组织中的表达,探讨其在草酸钙肾结石形成过程中的相关机制。方法:收集中南大学湘雅医院泌尿外科2015年4月至12月期间住院的30例草酸钙肾结石患者的肾乳头钙化斑组织标本作为实验组,10例同期患肾肿瘤行根治性肾切除术患者的肾乳头组织标本作为对照组,利用实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)和Western印迹检测MGP和BMP-2在肾乳头组织中的表达,并采用免疫组织化学技术观察MGP和BMP-2在肾乳头组织中的表达。结果:1)RT-PCR检测实验组和对照组MGP mRNA的表达量为0.760±0.804和1.365±0.348,差异有统计学意义(P0.05);实验组和对照组BMP-2 mRNA表达量分别为2.500±0.725和1.485±0.870,差异有统计学意义(P0.05)。2)Western印迹检测实验组和对照组的MGP蛋白表达量分别为0.130±0.424和0.202±0.704,差异无统计学意义(P0.05);实验组和对照组BMP-2蛋白表达量为0.885±0.220和0.682±0.272,差异有统计学意义(P0.05)。3)免疫组织化学结果显示实验组MGP的表达较对照组低,而实验组BMP-2表达较对照组高(均P0.05)。结论:草酸钙肾结石患者的肾乳头组织内BMP-2表达增多,MGP表达减少,草酸钙肾结石的形成可能是一种与MGP和BMP-2相关的类成骨反应或异位钙化。
[Abstract]:Objective: to compare the expression of matrix Gla protein and bone morphogenetic protein 2(bone morphogenetic protein 2 (BMP-2) in the calcified plaque of renal papilla of calcium oxalate stone, and to explore its mechanism in the formation of calcium oxalate nephrolithiasis. Methods: from April to December 2015, 30 specimens of renal papillary calcification spots of 30 patients with calcium oxalate nephrolithiasis were collected from the urology department of Xiangya Hospital of Central South University as experimental group (n = 10) with renal neoplasms undergoing radical nephrectomy. The specimens of renal papilla were used as control group. The expression of MGP and BMP-2 in renal papilla tissue was detected by real-time polymerase chain reactionation polymerase chain reaction (RT-PCR) and Western blot, and the expression of MGP and BMP-2 in renal papilla tissue was observed by immunohistochemistry. Results the expression of MGP mRNA in the experimental group and the control group was 0.760 卤0.804 and 1.365 卤0.348, the difference was statistically significant (P 0.05), the expression of BMP-2 mRNA in the experimental group and the control group was 2.500 卤0.725 and 1.485 卤0.870, respectively. The expression of MGP protein was 0.130 卤0.424 and 0.202 卤0.704, respectively, with no significant difference (P 0.05), but the expression of BMP-2 protein in experimental group and control group was 0.885 卤0.220 and 0.682 卤0.272, respectively. The immunohistochemical results showed that the expression of MGP in experimental group was lower than that in control group. The expression of BMP-2 in the experimental group was higher than that in the control group (P 0.05). Conclusion: the expression of BMP-2 in renal papilla of patients with calcium oxalate nephrolithiasis increased and decreased. The formation of calcium oxalate nephrolithiasis may be a kind of osteoid reaction or ectopic calcification associated with MGP and BMP-2.
【作者单位】：中南大学湘雅医院泌尿外科;
【基金】：中南大学中央高校基本科研业务费专项基金(2015zzts302)~~
【分类号】：R692.4

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