染料木黄酮对去势抵抗前列腺癌22RV1细胞增殖的影响

发布时间：2018-05-01 07:08

本文选题：染料木黄酮 + 去势抵抗前列腺癌　；参考：《郑州大学学报(医学版)》2017年04期

【摘要】：目的:探讨大豆异黄酮对去势抵抗前列腺癌细胞增殖的抑制作用及可能机制。方法:以不同浓度[0.0、12.5、25.0、50.0、100.0和(或)200.0μmol/L]的染料木黄酮处理去势抵抗前列腺癌细胞22RV1,在24、48、72 h采用CCK-8法测定细胞增殖情况,流式细胞术检测细胞周期,免疫细胞化学法检测Ki-67表达水平,Western blot法检测PSA、P53、CyclinD1、PCNA及AR表达水平。结果:染料木黄酮对22RV1细胞具有抑制作用。流式细胞术结果显示,染料木黄酮阻滞细胞周期于G2/M期(P0.05)。免疫细胞化学法检测结果显示,染料木黄酮能够抑制细胞中Ki-67的表达(P0.05)。Western blot结果显示染料木黄酮处理后细胞中CyclinD1、PCNA、PSA、AR的表达下降,P53的表达上调(P0.05)。结论:染料木黄酮能够抑制22RV1细胞的增殖,其作用机制可能与阻滞细胞G2/M期及下调周期蛋白CyclinD1表达有关。
[Abstract]:Aim: to investigate the inhibitory effect of soybean isoflavones on the proliferation of castrated prostate cancer cells and its possible mechanism. Methods: the castrated prostate cancer cell line 22RV1 was treated with genistein at different concentrations [0.012.525.0U 50.0100.0 and (or 200.0 渭 mol/L)]. The cell proliferation and cell cycle were measured by CCK-8 assay and flow cytometry at 2448.72 h, respectively. The expression level of Ki-67 was detected by immunocytochemistry and the expression of Ki-67 and AR by Western blot. Results: genistein inhibited 22RV1 cells. The results of flow cytometry showed that genistein blocked cell cycle at G _ 2 / M phase (P 0.05). The results of immunocytochemistry showed that genistein could inhibit the expression of Ki-67 in the cells. The results of Western blot showed that the expression of Cyclin D1 and PCNAgna PSAA AR was decreased and the expression of p53 was up-regulated in the cells treated with genistein. Conclusion: genistein can inhibit the proliferation of 22RV1 cells by blocking G2 / M phase and down-regulating the expression of cyclin CyclinD1.
【作者单位】：成都医学院公共卫生系卫生学教研室;
【基金】：国家自然科学基金资助项目81402678,81573154 四川省青年科技创新研究团队项目2014TD0021 成都医学院大学生创新实验项目201513705027
【分类号】：R737.25

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