芡实对糖尿病肾病大鼠肾组织GLUT1及TGFβ1表达的影响

发布时间：2018-05-08 00:18

本文选题：芡实 + 糖尿病肾病　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的：探讨芡实对糖尿病肾病大鼠肾组织GLUT1及TGFβ1表达的影响。方法：60只健康雄性SD大鼠随机分为正常对照组（N组）、糖尿病肾病组(DN)、芡实小剂量组(EL组)、芡实中剂量组（EM组）、芡实大剂量组（EH组）及氯沙坦钾组（LP组）。采用一次性腹腔注射链脲佐菌素（STZ,45mg/kg）制作DN模型，造模成功后，给予EL组大鼠芡实1.5g/kg/d，EM组大鼠芡实3.0g/kg/d，EH组大鼠芡实6.0g/kg/d，，LP组大鼠氯沙坦钾30mg/kg/d，N组及DN组给予等量蒸馏水，均采用灌胃给药。连续灌胃12周后检测大鼠血糖、肌酐、尿素氮、24小时尿蛋白定量；HE、Masson、PAS染色及电镜观察各组大鼠肾脏病理变化；免疫组化、western-blot法测定大鼠肾组织GLUT1和TGFβ1在表达情况。结果：1.生化指标结果：实验12周末，与正常组比较，血糖、BUN、Scr、24h尿蛋白定量显著升高（P0.05）；与DN组比较，芡实中、高剂量组大鼠病理改变减轻，BUN、Scr、24h尿蛋白定量显著下降（P0.05）；芡实中、高剂量组及氯沙坦钾组降尿蛋白作用无统计学差异。2.病理改变：与正常组比较，DN组大鼠可见肾小球肥大，系膜基质增生，基底膜增厚，芡实中、高剂量组及氯沙坦钾组大鼠上述病理改变较DN组减轻。3.肾组织GLUT1的表达：免疫组化结果显示，正常组大鼠GLUT1在肾小球系膜区和肾小管都有少量表达，DN组表达增强，以系膜区增强为主；与正常组比较，DN组GLUT1平均光密度值（IOD）显著升高（P0.05），与DN组比较，芡实中、高剂量组及氯沙坦钾组GLUT1的IOD明显降低（P0.05）；western-blot结果显示，与正常组比较，DN组GLUT1表达明显增强（P0.05），与DN组比较，芡实中、高剂量组及氯沙坦钾组GLUT1表达明显减弱（P0.05）；GLUT1在免疫组化、western-blot两种检测方法中表达一致。4.肾组织TGFβ1的表达：免疫组化结果显示，正常组大鼠TGFβ1在肾小球和肾小管上皮细胞都有少量表达，DN组表达增强，且以肾小管表达增强尤为显著；与正常组比较，DN组TGFβ1的IOD值显著升高（P0.05），与DN组比较，芡实中、高剂量组及氯沙坦钾组TGFβ1的IOD值明显降低（P0.05）；western-blot结果显示，与正常组比较，DN组TGFβ1表达明显增强（P0.05），与DN组比较，芡实中、高剂量组及氯沙坦钾组TGFβ1表达明显减弱（P0.05）；TGFβ1在免疫组化、western-blot两种检测方法中表达一致。结论：芡实可能通过下调GLUT1及TGFβ1在糖尿病肾病大鼠肾组织的表达，从而延缓糖尿病肾病的进展。
[Abstract]:Aim: to investigate the effect of Euryale cornifera on the expression of GLUT1 and TGF 尾 1 in renal tissue of diabetic nephropathy rats. Methods Sixty healthy male Sprague-Dawley rats were randomly divided into two groups: normal control group (n group), diabetic nephropathy group (group D), small dose group of Euryale thicken seed group (group EL), medium dose group of Euryale seed group (group EM), large dose group of Euryale seed group (group EH) and group of Losartan potassium group (group LP). The DN model was established by a single intraperitoneal injection of STZ (45 mg / kg). After the model was successfully established, the rats in the El group were given the same amount of distilled water. The rats in the EH group were fed with the same amount of distilled water at 3.0 g / kg 路kg ~ (-1) 路d ~ (th) of Loxartan potassium, 30 mg / kg 路kg ~ (-1) D ~ (-1) N and 30 mg / kg 路kg ~ (-1) 路d ~ (-1) N of losartan, respectively. Blood glucose, creatinine, urea nitrogen in 24 hours urine protein of rats were detected by intragastric perfusion for 12 weeks, and the renal pathological changes of each group were observed by electron microscope, and the expression of GLUT1 and TGF 尾 1 in renal tissue were detected by Western blot. The result is 1: 1. Results: at the end of the 12th week of the experiment, compared with the normal group, the quantity of urine protein in 24 hours of blood glucose was significantly increased, compared with DN group, the pathological changes of the high dose group decreased significantly (P0.05), the quantity of urine protein decreased significantly in the high dose group, and in the cornale seed group, the quantity of urine protein decreased significantly in the high dose group, and in the control group, there was no significant difference between the control group and the control group. There was no significant difference between high dose group and losartan potassium group. Pathological changes: compared with normal group, glomerular hypertrophy, Mesangial matrix hyperplasia, basement membrane thickening were observed in DN group. The expression of GLUT1 in renal tissue: immunohistochemical results showed that the expression of GLUT1 in glomerular Mesangial area and renal tubule of normal rats was increased slightly in DN group, mainly in Mesangial area. Compared with the normal group, the average optical density of GLUT1 in the DN group was significantly higher than that in the DN group. Compared with the DN group, the IOD of GLUT1 in the high dose group and the losartan potassium group decreased significantly compared with the normal group. The results showed that the GLUT1 expression in the DN group was significantly higher than that in the normal group, and that in the DN group was significantly higher than that in the DN group. The expression of GLUT1 in high dose group and losartan potassium group was significantly decreased in the two methods of immunohistochemical staining, I. E. the expression of GLUT1 was consistent with that of Losartan potassium group. Expression of TGF 尾 1 in renal tissue: immunohistochemical results showed that the expression of TGF 尾 1 in glomerular and tubular epithelial cells of normal rats was increased slightly in DN group, especially in renal tubules. Compared with the normal group, the IOD value of TGF 尾 1 in the DN group was significantly higher than that in the DN group. Compared with the DN group, the IOD value of TGF 尾 1 in the medium, high dose group and losartan potassium group decreased significantly. The results showed that the expression of TGF 尾 1 in the DN group was significantly higher than that in the normal group, and compared with that in the DN group, the expression of TGF 尾 1 in the cornstarch was higher than that in the DN group. The expression of TGF 尾 1 in high dose group and losartan potassium group was significantly decreased in both immunohistochemical and Western blot methods. Conclusion: Euryale may delay the progression of diabetic nephropathy by down-regulating the expression of GLUT1 and TGF 尾 1 in renal tissue of diabetic nephropathy rats.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R587.2;R692

【参考文献】