基于蛋白质组学的尿液中肾脏疾病相关蛋白的筛选

发布时间：2018-05-09 21:46

  本文选题：肾脏疾病 + 尿液　； 参考：《肾脏病与透析肾移植杂志》2015年03期

【摘要】：目的:通过对正常人血浆、尿液、肾组织及肾脏疾病患者尿液蛋白质组学获取的数据进行比较分析,寻找尿液中与肾脏疾病及肾脏损伤相关的生物标志物。方法:肾脏疾病患者尿液蛋白质组数据来自表现为肾病综合征的膜性肾病(MN)和局灶节段性肾小球硬化(FSGS)患者尿液蛋白质组谱图,从Peptide Atlas 2013数据库获得正常人血浆、尿液和肾组织蛋白质组数据。寻找肾脏疾病患者尿液蛋白质组相对正常人尿液蛋白质组富集蛋白,将筛选到的蛋白定义为肾脏疾病相关蛋白。寻找正常人血浆和尿液蛋白质组中不出现,同时在肾脏疾病患者尿液和肾组织蛋白质组中出现,且相对正常人尿液蛋白质组富集的蛋白,将筛选到的蛋白定义为肾损伤相关蛋白。结果:筛选到40种肾脏疾病相关蛋白。发现4种与肾损伤相关的蛋白,分别为泛素-60s核糖体蛋白L40、S相激酶相关蛋白1、转化生长因子βⅡ型受体、谷胱甘肽S转移酶A3。结论:通过对肾脏疾病患者尿液蛋白质组学数据与正常人血浆、尿液和肾组织的蛋白质组学数据进行比较分析,从患者尿液中获得了40种与肾脏疾病相关的蛋白和4种肾脏组织损伤相关的蛋白。上述蛋白成分在肾脏疾病诊断及病情判断中的意义有待进一步研究阐明。
[Abstract]:Objective: to find the biomarkers related to renal disease and renal injury by comparing and analyzing the proteomics data of normal human plasma, urine, renal tissue and renal diseases. Methods: the urine proteome data of patients with renal disease were obtained from the Peptide Atlas 2013 database of patients with membranous nephropathy (MNs) and focal segmental glomerulosclerosis (FSGS). Proteome data of urine and renal tissue. In order to find the proteome protein rich protein in urine of patients with renal disease, the selected protein is defined as the kidney disease related protein. It is found that there is no proteome in normal human plasma and urine, and it is also found in proteome of urine and renal tissue in patients with renal disease, and it is relatively rich in proteome in urine of normal people. The selected proteins are defined as kidney injury related proteins. Results: 40 kinds of renal disease related proteins were screened. Four proteins associated with renal injury were found to be ubiquitin-60 s ribosomal protein L40S phase kinase associated protein 1, transforming growth factor 尾-鈪，

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