HIF-1α及GLUT-1在前列腺癌中的表达及意义

发布时间：2018-05-12 08:00

本文选题：HIF-1α + GLUT-1　；参考：《新乡医学院》2014年硕士论文

【摘要】：背景前列腺癌(prostate cancer, PCa)是男性泌尿生殖系统常见肿瘤之一。目前对其治疗主要为手术及内分泌治疗。由于患者多为老年病人且早期临床症状不明显。因此,多数就诊时肿瘤已存在周边侵袭及远处转移的情况,进而失去根治性手术机会；内分泌治疗虽能够缓解病情,但无法从根本上治愈。因此,寻找特异性较高的列腺癌早期诊断方法已成为治疗前列腺癌的关键因素,同时也可能为前列腺癌的靶向治疗及判断预后提供新的参考。目的本研究以前列腺腺癌患者的癌组织及正常前列腺增生组织为研究对象,采用免疫组化方法检测前列腺腺癌组织中缺氧诱导因子HIF-1α及葡萄糖转运蛋白GLUT-1的表达水平,分析其表达水平与前列腺腺癌临床病理特征的关系；同时探索前列腺腺癌中缺氧诱导因子HIF-1α及葡萄糖转运蛋白GLUT-1的表达相关性,初步探讨它们在前列腺腺癌发生、发展中的作用及其临床应用价值。方法收集从2012年2月到2013年8月于新乡医学院第一附属医院泌尿外科手术切除前列腺腺癌标本46例,其中12例行耻骨后前列腺根治术；34例行经尿道前列腺电切术,标本均经病理学确诊为前列腺腺癌,术前均未经过化疗和放疗；另取40例前列腺增生组作为对照。组织采用多聚甲醛固定、脱水、石蜡包埋、3um连续切片,并烘干、脱蜡、水化、进行免疫组化(DAB法)染色,检测前列腺腺癌组织及前列腺增生组织中HIF-la及GLUT-1蛋白的表达情况,探讨HIF-1α、GLUT-1的表达水平与前列腺腺癌病理分级及临床分期的关系以及HIF-1α、GLUT-1蛋白在前列腺腺癌组织中的表达相关性。每个切片分别随机选取不重叠的5个高倍镜视野,分别由两名不知道切片分组的观察者,观察并计数阳性染色细胞数。采用AB值法(半定量法)进行相关分析。结果免疫组化DAB染色法结果显示,HIF-1α和GLUT-1蛋白在前列腺腺癌组织中的阳性表达率分别为60.9%和67.4%；前列腺增生组织中其阳性表达率分别为12.5%和17.5%,两者相比,HIF-1α和GLUT-1在前列腺腺癌中的表达显著增高,其差异具有统计学意义(P0.05); HIF-1α和GULT-1在前列腺癌中的表达与患者的临床病理分级及分期关系如下：在病理分级低分化、临床分期晚及伴有淋巴结及远处转移的标本中HIF-1α和GULT-1的阳性表达率明显高于病理高分化、临床分期早及无肿瘤转移者,其差异具有统计学意义(P0.05); HIF-1α和GLUT-1蛋白在前列腺腺癌组织中的阳性表达呈显著正相关关系(P0.05)。结论1.HIF-1α及GLUT-1在前列腺腺癌组织中呈正相关性高表达,且与肿瘤临床病理特征密相关,推测其可能在前列腺腺癌的发生及发展过程中起到至关重要的作用。 2. HIF-1α与GLUT-1可成为前列腺腺癌的生物行为学检测指标。
[Abstract]:Background Prostate cancer (PCA) is one of the most common tumors in male genitourinary system. At present, the main treatment is surgery and endocrine therapy. Because the patients are mostly elderly patients and early clinical symptoms are not obvious. Therefore, most of the tumors have been invasion and distant metastasis, and then lost the opportunity of radical surgery. Endocrine therapy can alleviate the disease, but can not be fundamentally cured. Therefore, the search for a highly specific method for early diagnosis of prostate cancer has become a key factor in the treatment of prostate cancer, and may also provide a new reference for the targeted treatment of prostate cancer and the judgment of prognosis. Purpose In this study, the expression of hypoxia inducible factor (HIF-1 伪) and glucose transporter (GLUT-1) in prostate adenocarcinoma tissues and normal prostatic hyperplasia tissues were detected by immunohistochemical method. To analyze the relationship between the expression level and clinicopathological features of prostate adenocarcinoma, and to explore the relationship between the expression of hypoxia inducible factor HIF-1 伪 and glucose transporter GLUT-1 in prostate adenocarcinoma, and to explore their occurrence in prostate adenocarcinoma. The role of development and its clinical application value. Method From February 2012 to August 2013, 46 cases of prostatic adenocarcinoma were treated by urological surgery in the first affiliated Hospital of Xinxiang Medical College. Among them, 12 cases were treated with retropubic radical prostatectomy and 34 cases with transurethral resection of prostate. All the specimens were pathologically diagnosed as prostatic adenocarcinoma without chemotherapy and radiotherapy before operation, and 40 cases of benign prostatic hyperplasia were taken as control group. The tissue was fixed with paraformaldehyde, dehydrated, paraffin embedded in 3um serial section, and dried, dewaxed, hydrated, and stained by immunohistochemical staining. The expression of HIF-la and GLUT-1 in prostate adenocarcinoma and prostatic hyperplasia was detected. To investigate the relationship between the expression of HIF-1 伪 -GLUT-1 and the pathological grade and clinical stage of prostate adenocarcinoma, and the correlation between the expression of HIF-1 伪 -GLUT-1 protein in prostate adenocarcinoma tissue. Each section randomly selected 5 high-power field of vision without overlapping. Two observers who did not know how to divide the sections were selected to observe and count the number of positive staining cells. AB value method (semi-quantitative method) was used for correlation analysis. Result The positive expression rates of HIF-1 伪 and GLUT-1 protein were 60.9% and 67.4% in prostate adenocarcinoma and 12.5% and 17.5% in benign prostatic hyperplasia, respectively. The positive expression rates of HIF-1 伪 and GLUT-1 in prostate gland were higher than that of HIF-1 伪 and GLUT-1 in prostate gland. There was a significant increase in expression in cancer, The relationship between the expression of HIF-1 伪 and GULT-1 in prostate cancer and the clinicopathological grades and stages of prostate cancer was as follows: low differentiation in pathological grade, low differentiation in pathological grade, The positive rates of HIF-1 伪 and GULT-1 in patients with late clinical stage and lymph node and distant metastasis were significantly higher than those with pathological differentiation. The positive expression of HIF-1 伪 and GLUT-1 protein in prostate adenocarcinoma was positively correlated with that of P0.05. Conclusion the expression of 1.HIF-1 伪 and GLUT-1 in prostate adenocarcinoma is positively correlated with the clinicopathological features of prostate adenocarcinoma. It is suggested that the expression of 1.HIF-1 伪 and GLUT-1 may play an important role in the occurrence and development of prostate adenocarcinoma. 2. HIF-1 伪 and GLUT-1 can be used as biomedical indicators for prostate adenocarcinoma.
【学位授予单位】：新乡医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25

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