骨桥蛋白与特发性草酸钙结石的相关性研究

发布时间：2018-05-12 22:39

本文选题：骨桥蛋白 + 特发性草酸钙结石　；参考：《南昌大学》2014年硕士论文

【摘要】：目的：通过观察特发性草酸钙结石（idiopathic calcium oxalate stone，ICS）患者肾组织中骨桥蛋白（osteopontin，OPN）mRNA和蛋白的表达水平及尿液中OPN浓度的变化，探讨OPN在ICS形成中的作用。方法：选取我院2013年1月至2014年1月期间因ICS致患肾无功能行肾切除术的34例肾组织标本为实验组，其中男性16例，女性18例，年龄13岁～73岁，平均50.7岁；收集同期行根治性肾切除术患者远癌正常肾组织标本38例作为对照组，其中男性16例，女性22例，年龄40岁～77岁，平均50.1岁。收集实验组和对照组患者尿液标本及19例正常人尿液标本（正常组）。采用Real-timeRT-PCR法检测两组肾组织中OPN mRNA的表达；Western blot法检测两组肾组织中OPN蛋白的表达情况；ELISA法测定三组尿液中OPN浓度。统计学数据用x±s表示，用SPSS14.0软件进行统计学处理，两组数据比较统计学分析采用两个独立样本的t检验，三组数据比较统计学分析采用单因素方差分析。结果：实验组与对照组肾组织OPN mRNA相对表达量ΔCT分别为2.04±1.20、2.59±0.91，差异有统计学意义（P0.05）；OPN蛋白的相对表达量分别为0.776±0.071、0.449±0.085，差异有统计学意义（P 0.05）；实验组、对照组、正常组尿液中OPN蛋白浓度分别为1.052±0.229、1.579±0.256、1.498±0.155，实验组和对照组、实验组与正常组相比，差异有统计学意义（P0.05），对照组与正常组相比，，差异无统计学意义（P0.05）。结论： ICS患者肾小管上皮细胞OPN mRNA和蛋白的过度表达及其尿液中OPN浓度的降低可能是肾结石形成的重要因素。
[Abstract]:Objective: To investigate the role of OPN in the formation of calcium oxalate by observing the expression level of osteopontinin in renal tissue and the concentration of OPN in urine in patients with idiopathic calcium oxalate stone (idiopathic calcium oxalate stone). Methods: From January 2013 to January 2014, 34 specimens of renal tissue were selected as experimental group, including 16 males and 18 females, aged from 13 to 73 years (mean 50.7 years). 38 cases of normal renal tissue of distal carcinoma were collected as control group, including 16 males and 22 females, aged from 40 to 77 years (mean 50.1 years). The urine samples of the patients in the experimental group and the control group and the urine samples of 19 normal persons (normal group) were collected. Real-timeRT-PCR method was used to detect the expression of OPN mRNA in renal tissues. Western blot method was used to detect the expression of OPN protein in renal tissues. Elisa was used to detect the concentration of OPN in urine of the three groups. The statistical data were expressed by x 卤s and processed by SPSS14.0 software. Two groups of data were compared and analyzed by t test of two independent samples, and three groups of data were compared by single factor analysis of variance. Results: The relative expression of OPN mRNA in the experimental group and the control group was 2.04 卤1.20 卤2.59 卤0.91respectively, and the relative expression of P0.05 was 0.776 卤0.071 + 0.449 卤0.085, respectively (P 0.05), the difference between the experimental group and the control group was significant (P 0.05), the difference between the experimental group and the control group was significant (P < 0.05). The concentration of OPN protein in urine of the normal group was 1.052 卤0.229 卤0.256 卤1.498 卤0.155, respectively. There was significant difference between the experimental group and the control group and between the experimental group and the normal group (P 0.05), but there was no significant difference between the control group and the normal group (P 0.05). Conclusion: The overexpression of OPN mRNA and protein in renal tubular epithelial cells and the decrease of OPN concentration in urine may be important factors in the formation of renal calculi in ICS patients.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R691.4

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