肾组织干细胞向肾小管上皮细胞的诱导分化

发布时间：2018-05-15 23:09

本文选题：肾组织干细胞 + 诱导分化　；参考：《南方医科大学学报》2015年02期

【摘要】：目的探讨肾组织干细胞向肾小管上皮细胞的诱导分化。方法分离肾乳头肾组织干细胞,对照组加入正常干细胞培养基,诱导组应用含activin A、BMP-7、维甲酸3种细胞因子的诱导培养基与肾上皮细胞培养基交替诱导,同时与缺氧损伤的肾小管上皮细胞共培养,通过细胞流式、免疫荧光、q RT-PCR的方法评估诱导效率。结果细胞流式结果提示,对照组肾组织干细胞CK-18阳性率为6.5%,诱导组CK-18阳性率为44.2%;免疫荧光结果提示,诱导组成熟上皮细胞标记CK-18、E-cadherin、ZO-1部分阳性表达;q RT-PCR结果提示,诱导组成熟上皮细胞标记E-cadherin、AQP-1基因表达水平较对照组显著升高(P0.01)。结论肾组织干细胞在体外可以诱导分化为肾小管上皮细胞。
[Abstract]:Objective to investigate the differentiation of renal stem cells into renal tubular epithelial cells. Methods Renal papillary renal stem cells were isolated, and the normal stem cell medium was added to the control group. In the induction group, three kinds of cytokines containing activin Agna BMP-7 and retinoic acid were used in the induction medium alternately with the renal epithelial cell medium. The induction efficiency was evaluated by flow cytometry and immunofluorescence Q RT-PCR. Results the CK-18 positive rate of renal stem cells in the control group was 6.5 and the positive rate of CK-18 in the induced group was 44.2. The immunofluorescence results showed that the positive rate of CK-18E-cadherinine ZO-1 partial expression in the mature epithelial cells of the induced group was similar to that of the CK-18E-cadherinine ZO-1. Compared with the control group, the expression of E-cadherinine AQP-1 gene in the mature epithelial cells in the induction group was significantly higher than that in the control group (P 0.01). Conclusion Renal stem cells can be induced to differentiate into renal tubular epithelial cells in vitro.
【作者单位】：中国人民解放军总医院南楼肾脏病科肾脏疾病国家重点实验室;军事医学科学院干细胞与再生医学实验室;
【基金】：国家自然科学基金(81170312,30772296) 北京市自然科学基金(7122163) 解放军总医院扶持基金(2012FC-CXYY-4005)~~
【分类号】：R692.6

【参考文献】