KLF5和TAZ在膀胱癌组织中的表达及临床意义

发布时间：2018-05-16 07:00

本文选题：膀胱癌 + TAZ　；参考：《天津医科大学》2014年硕士论文

【摘要】：目的：膀胱癌在泌尿系统肿瘤中的发病率和死亡率均居首位,具有难检测、易复发、预后差等特点。膀胱癌的发病是多因素、多阶段相互作用的复杂过程,其发病机制迄今尚未明确,因此仍缺乏有效的诊断、预防和治疗措施。KLF5是近年发现的一种锌指蛋白转录因子,能够通过抑制或激活目的基因的转录调控基因表达,在细胞增殖、分化、凋亡和肿瘤的发生发展中都发挥重要作用。TAZ是一种转录共激活因子,能够与多种调控因子相互作用,促进癌细胞的增殖和肿瘤的发生,KLF5转录因子可能与TAZ的WW结构域相互作用促进癌细胞的增殖和肿瘤的形成。本研究通过检测KLF5和TAZ在膀胱癌及正常膀胱组织中的表达,分析其与膀胱癌分化程度、病理分级、TNM分期等临床病理参数的相关性以及两者之间的相关性。方法：应用免疫组织化学方法检测46例膀胱癌及10例正常膀胱组织中KLF5和TAZ的表达水平,分析两者与膀胱癌分化程度、病理分级TNM分期等临床病理参数的相关性以及两种转录调控因子之间的相关性。结果：膀胱癌组织中KLF5和TAZ的阳性表达率都高于正常膀胱组织,膀胱癌组织中KLF5的阳性表达率为78.3%(36/46),TAZ的阳性表达率为76.1%(35/46),与正常膀胱组织中的KLF5和TAZ表达量相比,都具有显著性差异(P0.05)。KLF5和TAZ蛋白表达与肿瘤的分化程度(P=0.023；P=0.008)和病理分级(P=0.025；P=0.016)显著相关,与性别、病理TNM分期和肿瘤转移无显著相关性。结论：膀胱癌组织中的TAZ和KLF5的表达上调与肿瘤的生长、分化、浸润和预后都密切相关,表明TAZ和KLF5是膀胱癌发生发展和患者预后优劣的重要标志物,能够为膀胱癌的发生和癌变提供一个诊断指标,为今后的临床研究提供重要的理论依据。
[Abstract]:Objective: the incidence and mortality of bladder cancer are the first in urological tumor. It has the characteristics of difficult detection, recurrence and poor prognosis. The pathogenesis of bladder cancer is a complex process of multiple factors and multistage interaction. The pathogenesis of bladder cancer is not clear so far. Therefore, the prevention and treatment measures of.KLF5 are still found in recent years. A zinc finger protein transcription factor, which can play an important role in cell proliferation, differentiation, apoptosis and tumor development by inhibiting or activating transcriptional regulation of gene expression in the target gene..TAZ is a transcriptional co activating factor, which can interact with a variety of regulators to promote the proliferation of cancer cells and the occurrence of tumors, KLF The 5 transcriptional factor may interact with the WW domain of TAZ to promote the proliferation of cancer cells and the formation of tumor. This study examined the expression of KLF5 and TAZ in bladder cancer and normal bladder tissue, and analyzed the correlation with the degree of differentiation, pathological grade, TNM staging and other clinicopathological parameters, and the correlation between them.
Methods: the expression of KLF5 and TAZ in 46 cases of bladder cancer and 10 normal bladder tissues was detected by immunohistochemistry. The correlation between the differentiation degree of bladder cancer, the clinicopathological parameters, such as the pathological grade TNM staging, and the correlation between the two kinds of transcription factors were analyzed.
Results: the positive expression rate of KLF5 and TAZ in bladder cancer tissues is higher than that of normal bladder tissue. The positive expression rate of KLF5 in bladder cancer tissues is 78.3% (36/46), and the positive expression rate of TAZ is 76.1% (35/46). Compared with the expression of KLF5 and TAZ in normal bladder tissue, there are significant differences (P0.05).KLF5 and TAZ protein expression and tumor score. The degree of P=0.023 (P=0.008) and pathological grading (P=0.025; P=0.016) were significantly correlated with gender, pathological TNM stage and tumor metastasis.
Conclusion: the up-regulated expression of TAZ and KLF5 in bladder cancer tissues is closely related to the growth, differentiation, infiltration and prognosis of the tumor. It shows that TAZ and KLF5 are important markers for the development of bladder cancer and the prognosis of the patients. It can provide a diagnostic index for the occurrence and canceration of bladder cancer and provide an important theory for future clinical research. Basis.

【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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