糖尿病肾病大鼠模型肾小管间质纤维化与瘀血阻络的相关性分析

发布时间：2018-05-16 18:51

本文选题：糖尿病肾病 + 肾小管间质纤维化　；参考：《时珍国医国药》2017年02期

【摘要】：目的探讨糖尿病肾病大鼠模型肾小管间质纤维化与瘀血阻络的相关性。方法 30只SD大鼠随机平分为三组-瘀血阻络型组(A组)、非瘀血阻络型组(B组)、正常组(C组),均予高糖高脂饲料喂养,A组与B组采用一次性腹腔注射链脲佐菌素(STZ)诱导建立糖尿病肾病模型,观察大鼠行为,分别测定随机血糖、24h尿微量白蛋白含量;取大鼠的肾皮质,病理切片染色观察肾小管间质的变化;也采用western blot检测各组大鼠肾组织TGF-β1的表达情况。结果 A组与B组的不同时间点的血糖明显高于C组(P0.05),A组与B组的组间对比无明显差异(P0.05)。A组与B组的24h尿微量白蛋白定量分别为(24.20±4.10)mg/24h和(24.29±3.89)mg/24h,都明显高于C组的(5.93±2.14)mg/24h(P0.05)。A组与B组的TGF-β1表达量明显高于C组(P0.05),同时A组的TGF-β1表达量也高于B组(P0.05)。形态学观察显示相对于B组,A组的肾小球体积增大,基底膜增厚,系膜基质增多。结论糖尿病肾病大鼠模型中瘀血阻络型的TGF-β1表达量相对比较高,伴随有肾小管间质纤维化,可导致大鼠病情恶化,也确认了糖尿病肾病瘀血阻络型的存在。
[Abstract]:Objective to investigate the correlation between tubulointerstitial fibrosis and blood stasis in diabetic nephropathy rats. Methods Thirty Sprague-Dawley rats were randomly divided into three groups: group A of blood stasis blocking collaterals, group B of non-blood stasis blocking collaterals, and group C of normal control. All of them were fed with high sugar and high fat diet, group A and group B were given intraperitoneal injection of streptozotocin. The model of diabetic nephropathy was induced by STZ. The changes of renal tubulointerstitium were observed by pathological section staining, and the expression of TGF- 尾 1 in renal tissue of each group was also detected by western blot. Results the blood glucose levels in group A and group B were significantly higher than those in group C and group B at different time points. There was no significant difference in comparison between group A and group B. The levels of 24 h urinary microalbumin in group A and group B were 24.20 卤4.10)mg/24h and 24.29 卤3.89 mg / 24 h, respectively, which were significantly higher than those in group C (5.93 卤2.14)mg/24h(P0.05).A). The expression of TGF- 尾 1 in group B was significantly higher than that in group C, and the expression of TGF- 尾 1 in group A was higher than that in group B. Compared with group B, the glomerular volume, basement membrane thickening and Mesangial matrix increased. Conclusion the expression of TGF- 尾 1 in the rat model of diabetic nephropathy is relatively high, accompanied by renal tubulointerstitial fibrosis, which can lead to the deterioration of the condition of the rat, and confirms the existence of the blood stasis type of diabetic nephropathy.
【作者单位】：河北中医学院基础医学院;河北省中医院(河北中医学院附属医院);河北中医学院针灸推拿学院;河北医科大学;
【基金】：国家自然科学基金(No.81373804) 河北中医学院博士基金课题项目(No.BSZ2014004)
【分类号】：R587.2;R692.9;R-332

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