盐霉素对人膀胱癌5637细胞的生长抑制作用

发布时间：2018-05-17 00:14

本文选题：盐霉素 + 膀胱肿瘤　；参考：《吉林大学学报(医学版)》2014年03期

【摘要】：目的:探讨盐霉素对人膀胱癌5637细胞生长、凋亡和侵袭力的影响,阐明其可能的作用机制。方法:取体外培养的对数生长期人膀胱癌5637细胞,分为空白对照组和不同剂量(15、30和60μmol·L-1)盐霉素组。MTT比色法检测各组人膀胱癌5637细胞的生长抑制率,流式细胞术检测各组人膀胱癌5637细胞的细胞凋亡率,Matrigel侵袭实验检测各组人膀胱癌5637细胞的侵袭能力,Western蛋白印迹实验检测各组人膀胱癌5637细胞中Wnt/β-catenin途径β-catenin蛋白表达水平。结果:与空白对照组比较,各剂量盐霉素组膀胱癌5637细胞的生长抑制率明显升高(P0.05),细胞凋亡率明显升高(P0.05),细胞穿膜数目减少(P0.05),β-catenin蛋白表达水平减少(P0.05)。与低剂量(15μmol·L-1)盐霉素组比较,高剂量(60μmol·L-1)盐霉组人膀胱癌5637细胞生长抑制率升高(P0.05),细胞凋亡率升高(P0.05),细胞穿膜数目减少(P0.05),β-catenin蛋白表达量减少(P0.05)。结论:盐霉素对人膀胱癌5637细胞的生长有较明显抑制作用,促进细胞凋亡,降低细胞侵袭力,并且这种抑制作用可能是通过抑制Wnt/β-catenin信号传导途径来实现的。
[Abstract]:Aim: to investigate the effects of salinomycin on the growth, apoptosis and invasion of human bladder cancer cell line 5637, and to elucidate its possible mechanism. Methods: 5637 human bladder cancer cells cultured in logarithmic phase in vitro were divided into two groups: control group and different dose group (1530 and 60 渭 mol L-1). MTT colorimetric assay was used to detect the growth inhibition rate of human bladder cancer 5637 cells in each group. Apoptosis rate and Matrigel invasion assay of human bladder cancer 5637 cells were detected by flow cytometry. Western blot assay was used to detect the expression of Wnt/ 尾 -catenin pathway 尾 -catenin protein in human bladder cancer cell line 5637. Results: compared with the control group, the growth inhibition rate, apoptosis rate, cell number and 尾 -catenin protein expression of 5637 cells were significantly increased, P0.05, P0.05, P0.05 and 尾 -catenin protein expression levels were significantly increased in different doses of salinomycin group compared with the control group. Compared with the low dose (15 渭 mol L -1) salinomycin group, the growth inhibition rate, apoptosis rate, cell number and 尾 -catenin protein expression of 5637 cell line were increased, P0.05, P0.05, P0.05and 尾 -catenin protein expression were decreased in high dose 60 渭 mol L-1 group. Conclusion: salinomycin can inhibit the growth of human bladder cancer cell line 5637, promote cell apoptosis and decrease cell invasion, and this inhibition may be achieved by inhibiting Wnt/ 尾 -catenin signal transduction pathway.
【作者单位】：吉林大学第一医院泌尿外科;吉林大学第一医院乳腺外科;
【基金】：吉林省卫生厅科研基金资助课题(2008Z060) 吉林大学白求恩计划B项目基金资助课题(2012217)
【分类号】：R737.14

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