侧群细胞与肾癌肿瘤干细胞研究进展

发布时间：2018-05-17 11:27

本文选题：肾肿瘤 + 肿瘤干细胞　；参考：《中华肿瘤防治杂志》2015年14期

【摘要】：目的总结国内外侧群细胞(side population,SP)及其在肾癌肿瘤干细胞中的研究进展。方法应用PubMed及中国知网期刊全文数据库检索系统,以"肿瘤干细胞、侧群细胞和肾癌"等为关键词,检索1996-01-2015-01的相关文献,共检索到英文文献660条,中文文献1 041条。纳入标准:1)肿瘤干细胞生物学特性;2)SP的表面标志和分选方法;3)肾癌肿瘤干细胞与SP相关性的研究。剔除标准:重复研究。最后纳入分析文献39篇。结果肿瘤干细胞是肿瘤发生、生长、转移和复发的根源,SP存在多种肿瘤细胞及肿瘤组织中,具有自我更新、多向分化和强大致瘤能力等干细胞的生物学特性,是肿瘤干细胞富集的亚群。利用SP细胞分选技术分离鉴定肿瘤干细胞及研究其生物学功能,将为肿瘤干细胞的研究提供新的方向。在对肾癌肿瘤干细胞的研究中发现SP细胞的分布,SP细胞表达的腺苷三磷酸转运蛋白G2(ATP binding cassette,ABCG2)能将多种化疗药物和外源性化学物质排出胞外,它的这种特性被认为与肾癌的多药耐药密切相关;肾癌SP细胞中乙醛脱氢酶1(aldehyde dehydrogenase 1,ALDH1)的表达明显高于非SP细胞,ALDH1阳性细胞在成球能力及致瘤能力上明显高于ALDH1阴性细胞。但是无论是ABCG2还是ALDH1都不是形成SP细胞表型的唯一原因。结论 SP可用于肾癌肿瘤干细胞的分选,但肾癌肿瘤干细胞的表面特征性标志仍需进一步研究。
[Abstract]:Objective to summarize the research progress of lateral cell side population (SPV) and its application in renal cell carcinoma (RCC) stem cells at home and abroad. Methods by using PubMed and the full text database retrieval system of Chinese Journal of Zhiwang, the relevant literatures of 1996-01-2015-01 were searched with the key words of "tumor stem cells, lateral group cells and renal cell carcinoma". A total of 660 articles in English and 1,041 articles in Chinese were retrieved. Study on the relationship between tumor Stem cells and SP in RCC. Exclusion criteria: repeated studies. Finally, 39 papers were included in the analysis. Results tumor stem cells are the root of tumor genesis, growth, metastasis and recurrence. There are many kinds of tumor cells and tumor tissues, which have the biological characteristics of self-renewal, multi-differentiation and strong ability of tumorigenesis. It is a rich subgroup of tumor stem cells. The isolation and identification of tumor stem cells and their biological functions by SP cell sorting will provide a new direction for the study of tumor stem cells. The distribution of SP cells and the expression of adenosine triphosphate transporter (G2(ATP binding cassettein ABCG2) were found in the study of tumor stem cells of renal cell carcinoma (RCC), and many chemotherapeutic drugs and exogenous chemicals could be excreted out of the cells by the expression of adenosine triphosphate transporter (G2(ATP binding cassettein ABCG2). The expression of acetaldehyde dehydrogenase (1(aldehyde dehydrogenase _ 1) and ALDH _ 1 in SP cells was significantly higher than that in ALDH1 negative cells. But neither ABCG2 nor ALDH1 is the only reason for SP cell phenotype. Conclusion SP can be used in the sorting of renal cancer tumor stem cells, but the surface characteristic markers of renal cancer tumor stem cells need to be further studied.
【作者单位】：山东医学高等专科学校外科教研室;
【基金】：山东省高等学校科技计划(J13LL08) 临沂市科技发展计划(201515055)
【分类号】：R737.11

【参考文献】