CD147及MMP-2在前列腺癌组织中的相关性研究

发布时间：2018-05-20 21:37

本文选题：CD147 + MMP-2　；参考：《天津医科大学》2014年硕士论文

【摘要】：背景与目的：前列腺癌(Prostate carcinoma,PCa)是男性泌尿系统中比较常见的恶性肿瘤,在世界范围内的发病率占男性所有恶性肿瘤的第二位,是西方国家最常见的泌尿系统肿瘤,在我国的发病率也呈现出逐年上升的趋势。外科手术或者内分泌治疗是目前最为有效的治疗措施,但仍容易复发和转移,严重影响患者的预后生存。前列腺癌的发病机制至今仍未阐明。因其临床症状较为隐匿、自然病程也较长,普查未完全普及,许多的患者确诊时已发展到了晚期。近年来研究证明,CD147、MMP-2与肿瘤的发生和发展存在着密切的关系,本实验通过免疫组化的方法检测CD147和MMP-2在前列腺癌组织中表达,分析CD147和MMP-2在前列腺癌癌组织中的表达与肿瘤的分化程度、临床分期、有无转移、年龄、PSA等因素之间的关系及其相关性。探讨两者在前列腺癌的发生以及发展过程中作用,为临床诊断及监测预后提供参考依据。资料与方法：选取天津医科大学第二医院2010年3月-2013年3月间前列腺疾病的石蜡包埋标本90例,其中前列腺癌标本60例,年龄58-81岁,平均67岁；良性前列腺增生标本30例,年龄55-82岁,平均65岁。两组间年龄差异无统计学意义。根据前列腺癌危险因素等级再进行分组,按Gleason分级低中危(2-7分)24例,高危(8-10分)36例；按TNM分期,T1-T2b期29例,T2c-T4期31例；PSA≤20ng/ml25例,PSA20ng/m135例；发生淋巴结转移者32例,无淋巴结转移者28例。所有患者术前都没有接受放疗、化疗或者内分泌治疗。实验采用链霉菌素抗生物素蛋白-过氧化物酶免疫组化法(streptavidin-peroxidase, SP法)进行检测。染色步骤按照试剂盒的说明书分别进行,选用乳腺癌已知MMP-2和CD147阳性组织切片作为阳性对照,用PBS液代替一抗作为阴性对照。MMP-2阳性表达主要表现为在细胞浆出现棕色或者棕黄色颗粒,CD147阳性表达主要表现为在细胞膜或细胞浆中出现棕色或者棕黄色颗粒,用阳性细胞的标记个数结合染色强度来综合判断染色结果。应用SPSS19.0统计软件包对所有数据进行统计学分析。P0.05代表差异有统计学意义。结果：(1)在60例前列腺癌组织中,有41例呈CD147的阳性表达,阳性表达率为68.3%；而在30例良性前列腺增生的组织中,有4例呈阳性表达,阳性表达率为13.3%。前列腺癌组和良性前列腺增生组进行比较,差异具有统计学意义,P0.01。CD147在前列腺癌细胞中的阳性表达率显著高于良性前列腺增生组。(2)在60例前列腺癌组织中,有46例呈MMP-2的阳性表达,阳性表达率为76.7%；而在30例良性前列腺增生组织中,阳性表达有3例,阳性率为10.0%。前列腺癌组和良性前列腺增生组进行比较,差异有统计学意义,P0.01。MMP-2在前列腺癌细胞中的阳性表达率显著高于良性前列腺增生组。(3)在60例前列腺癌组织中,MMP-2和CD147的阳性表达与患者的PSA和年龄无关,其阳性表达率在不同PSA和不同年龄患者中差异无统计学意义,P0.05。但是在不同的病理分级、临床分期及有无转移的患者中表达具有统计学意义,P0.05。有转移的患者标本MMP-2和CD147阳性表达率要高于无转移的患者；Glesaon评分高及TNM分期高的患者中MMP-2和CD147阳性表达率也较高。(4)在60例前列腺癌组织中,其中有37例同时表达MMP-2和CD147,而同为阴性者有10例,经Spearman相关分析表明,MMP-2和CD147在前列腺癌组织中表达呈正相关(r=0.472,P0.01) 结论：(1)研究显示CD147和MMP-2在前列腺癌组织中的表达明显上调,且与良性前列腺增生组织相比较差异有统计学意义,提示CD147和MMP-2可能参与前列腺癌的发生。(2)CD147和MMP-2在前列腺癌组织中的表达与肿瘤的病理分级、临床分期和是否转移密切相关,与年龄及PSA无关。CD147和MMP-2高表达者其肿瘤的恶性程度也较高,侵袭性较强,更具有转移的倾向。提示CD147和MMP-2可能参与前列腺癌的进展以及扩散转移。(3)在前列腺癌组织中,CD147和MMP-2的表达呈显著正相关,两者可能在前列腺癌的发生、发展及转移过程中起着协同作用。(4)CD147和MMP-2蛋白表达的联合检测可以对前列腺癌的发生、发展、预后及药物治疗提供重要的参考依据。
[Abstract]:Background and purpose: Prostate carcinoma (PCa) is the most common malignant tumor in the male urinary system. The incidence of the disease in the world accounts for second of all male malignant tumors. It is the most common urological tumor in the western countries, and the incidence of the disease is also rising year by year in our country. Secretory therapy is the most effective treatment at present, but it is still prone to recurrence and metastasis, which seriously affects the survival of the patients. The pathogenesis of prostate cancer has still not been clarified. Because of its clinical symptoms are concealed, the natural course is long, the general survey is not completely popularized, and many patients have been developed to the late stage. In recent years, the research has proved that CD147 and MMP-2 are closely related to the development and development of tumors. In this experiment, the expression of CD147 and MMP-2 in prostate cancer tissues was detected by immunohistochemical method. The expression of CD147 and MMP-2 in the cancer tissues of the prostate and the degree of differentiation of the tumor, the clinical stages, the relationship between the metastasis, age, and PSA and other factors were analyzed. Objective to explore the role of both in the occurrence and development of prostate cancer, so as to provide reference for clinical diagnosis and prognosis.
Materials and methods: 90 cases of paraffin embedded specimens of prostate disease in Second Hospital Affiliated to Tianjin Medical University in March -2013 March 2010 were selected, including 60 cases of prostate cancer, 58-81 years old and 67 years old, 30 cases of benign prostatic hyperplasia, 55-82 years old, with an average of 65 years. The age difference between the two groups was not statistically significant. According to the prostate cancer, the prostate cancer was not statistically significant. Risk factors were divided into groups, 24 cases of low medium risk (2-7 points) and 36 cases at high risk (8-10 points) according to Gleason classification, TNM staging, 29 cases of T1-T2b, 31 cases of T2c-T4, PSA < 20ng/ml25, PSA20ng/m135 cases, 32 cases of lymph node metastasis, and 28 cases without lymph node metastasis. All patients were not treated with radiotherapy, chemotherapy, or endocrinology before operation. The test was carried out by Streptomyces anti biotin peroxidase immunohistochemical method (streptavidin-peroxidase, SP). The staining steps were carried out according to the instructions of the kit, and the positive control of the breast cancer MMP-2 and CD147 positive tissue sections was selected, and the PBS solution was used instead of the negative control.MMP-2 positive table. The main manifestations were brown or brown granules in cytoplasm. The positive expression of CD147 was brown or brown granules in cell membrane or cytoplasm. The staining results were judged by the number of positive cells combined with the staining intensity. All data were statistically analyzed by the SPSS19.0 software package. P0.05 represents a statistically significant difference.
Results: (1) in 60 cases of prostate cancer, 41 cases showed positive expression of CD147 and the positive expression rate was 68.3%. In 30 cases of benign prostatic hyperplasia, 4 cases showed positive expression, the positive rate was compared with the 13.3%. prostate cancer group and the benign prostatic hyperplasia group, the difference was statistically significant, P0.01.CD147 was in the prostate. The positive expression rate in the cancer cells was significantly higher than that in the benign prostatic hyperplasia group. (2) in 60 cases of prostate cancer, 46 cases showed positive expression of MMP-2 and the positive expression rate was 76.7%. In 30 cases of benign prostatic hyperplasia, the positive expression was 3 cases, the positive rate was compared with the anterior 10.0%. adenocarcinoma group and the benign prostatic hyperplasia group. The positive expression rate of P0.01.MMP-2 in the prostate cancer cells was significantly higher than that in the benign prostatic hyperplasia group. (3) in 60 cases of prostate cancer, the positive expression of MMP-2 and CD147 was not related to the PSA and age of the patients. The positive expression rate was not statistically significant in different PSA and different years of age, but P0.05. was not significant. The expression of the same pathological grading, clinical staging and non metastasis was statistically significant. The positive expression rate of MMP-2 and CD147 in patients with P0.05. metastasis was higher than that of non metastatic patients; the positive expression rate of MMP-2 and CD147 in patients with high Glesaon score and high TNM stage were also higher. (4) among 60 cases of prostate cancer, 37 MMP-2 and CD147 were expressed at the same time, and 10 cases were negative. The Spearman correlation analysis showed that the expression of MMP-2 and CD147 in the prostate cancer tissues was positively correlated (r=0.472, P0.01).
Conclusions: (1) the study showed that the expression of CD147 and MMP-2 in the prostate cancer tissues was significantly up-regulated and compared with the benign prostatic hyperplasia, suggesting that CD147 and MMP-2 may be involved in the occurrence of prostate cancer. (2) the expression of CD147 and MMP-2 in the prostate cancer tissues and the pathological classification of the tumor, clinical stages and whether or not. Metastasis is closely related,.CD147 and high expression of PSA are not related to age and MMP-2, with higher malignancy, stronger invasiveness and more metastatic tendency. It suggests that CD147 and MMP-2 may be involved in the progression of prostate cancer and metastasis. (3) in prostate cancer, the expression of CD147 and MMP-2 is positively correlated, and both may be in the front of the prostate cancer. There is a synergistic effect in the occurrence, development and metastasis of adenocarcinoma. (4) the combined detection of CD147 and MMP-2 protein expression can provide important reference for the development, development, prognosis and drug treatment of prostate cancer.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.25

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