老年大鼠肾脏损伤中ASPP2蛋白调控与内质网应激的作用及相关性分析

发布时间：2018-05-26 23:15

本文选题：P凋亡刺激蛋白 + 内质网应激　；参考：《广东医学》2017年16期

【摘要】：目的探讨P53凋亡刺激蛋白(ASPP)家族成员ASPP2蛋白及内质网应激在老年大鼠肾脏损伤中的作用机制。方法以6月龄SD大鼠为成年组,18月龄SD大鼠为老年组,苏木精-伊红染色(HE)观察各组大鼠肾脏结构的改变;qRT-PCR检测内质网应激相关指标C/EPB同源蛋白(CHOP)、活化转录因子6(ATF6)、X盒结合蛋白-1(XBP-1)、葡萄糖调节蛋白78(GRP78)的mRNA表达水平;Western blot法检测各组大鼠ASPP2蛋白的表达水平,并对ASPP2的表达水平与内质网应激相关指标CHOP、ATF6、XBP-1、GRP78的表达水平作相关性分析。结果与成年组SD大鼠相比,老年组SD大鼠肾脏结构明显改变,CHOP、ATF6、XBP-1、GRP78的mRNA表达水平及ASPP2蛋白表达水平显著增高,且ASPP2水平与CHOP和XBP-1呈正相关,ASPP2水平与ATF6呈负相关。结论在老年SD大鼠中,ASPP2调控内质网应激可能是引起肾脏损伤的重要机制之一。
[Abstract]:Objective to investigate the role of ASPP2 protein and endoplasmic reticulum stress (ER) in renal injury in aged rats. Methods Sprague-Dawley rats aged 6 months were used as adult group and 18 months old SD rats as aged group. The changes of renal structure in rats were observed by hematoxylin and eosin staining. The expression level of C/EPB homologous protein (C/EPB homologous protein), the activation transcription factor 6 (ATF6) X box binding protein-1 (XBP-1) and the glucose-regulated protein 78 (GRP78) were detected by mRNA blot method in each group. The expression level of ASPP2 protein was detected in each group. Correlation analysis was made between the expression level of ASPP2 and the expression level of the endoplasmic reticulum stress related index CHOPF6 XBP-1GRP78. Results compared with the adult SD rats, the renal structure of SD rats in the aged group was significantly changed. The expression of mRNA and ASPP2 protein were significantly increased in the rat kidney of CHOPP-ATF6, XBP-1, GRP78, and the level of ASPP2 was positively correlated with CHOP and XBP-1 and negatively correlated with ATF6. Conclusion the regulation of endoplasmic reticulum stress by ASPP2 may be one of the important mechanisms of renal injury in aged SD rats.
【作者单位】：宁夏医科大学基础医学院;四川大学华西基础医学与法医学院;
【基金】：国家自然科学基金资助项目(编号:81360063,81460121)
【分类号】：R692

【相似文献】