血小板衍化生长因子DD刺激大鼠肾成纤维细胞增殖及向肌成纤维细胞的转化

发布时间：2018-05-29 13:21

本文选题：肾 + 纤维化　；参考：《中国组织工程研究》2015年11期

【摘要】：背景:血小板衍化生长因子可诱导肾小球系膜细胞及肾间质多种细胞增殖、迁移、转化及细胞外基质的过表达。目的:观察血小板衍化生长因子DD/血小板衍化生长因子βR信号通路激活对大鼠肾成纤维细胞细胞增殖及α-平滑肌肌动蛋白表达的影响。方法:体外培养正常大鼠肾成纤维细胞(NRK-49F),按照血小板衍化生长因子DD刺激质量浓度分为对照组、1μg/L组、10μg/L组、50μg/L组、100μg/L组,然后根据50μg/L血小板衍化生长因子DD刺激不同时间分为对照组、12 h组、24 h组、48 h组。CCK8检测不同血小板衍化生长因子DD质量浓度及血小板衍化生长因子DD作用不同时间对NRK-49F增殖的影响;Real-time PCR检测各质量浓度组血小板衍化生长因子βR、α-平滑肌肌动蛋白m RNA变化;Western blot检测各浓度组血小板衍化生长因子βR、α-平滑肌肌动蛋白蛋白表达变化。结果与结论:与对照组相比,血小板衍化生长因子DD可明显刺激NRK-49F细胞增殖,呈现剂量依赖性及时间依赖性。血小板衍化生长因子DD可剂量依赖性刺激其相应血小板衍化生长因子βR及α-平滑肌肌动蛋白在m RNA水平及蛋白水平上的表达增加。提示血小板衍化生长因子DD/血小板衍化生长因子βR信号通路的激活可显著刺激NRK-49F细胞的增殖及向肌成纤维细胞的转化。
[Abstract]:Background: platelet-derived growth factor can induce proliferation, migration, transformation and overexpression of glomerular Mesangial cells and renal interstitial cells. Aim: to investigate the effects of platelet derived growth factor (DD/) on proliferation and 伪 -smooth muscle actin expression in rat renal fibroblasts by activation of platelet derived growth factor 尾 R signaling pathway. Methods: normal rat renal fibroblasts were cultured in vitro and divided into 10 渭 g / L control group and 100 渭 g / L group according to platelet derived growth factor DD stimulation concentration. Then according to 50 渭 g / L platelet derived growth factor DD stimulation time, divided into control group 12 h group 24 h group 48 h group. CCK8 detection of different platelet-derived growth factor DD concentration and platelet derived growth factor DD effect at different times The effect of real-time PCR on the proliferation of platelet derived growth factor 尾 R, 伪 -smooth muscle actin m RNA and the expression of platelet derived growth factor 尾 R and 伪 -smooth muscle actin in different concentration groups were detected by real-time PCR. Results and conclusion: platelet derived growth factor DD could stimulate the proliferation of NRK-49F cells in a dose-and time-dependent manner. Platelet derived growth factor DD stimulated the expression of platelet derived growth factor 尾 R and 伪 -smooth muscle actin at m RNA level and protein level in a dose-dependent manner. It is suggested that the activation of platelet derived growth factor (DD/) -derived growth factor 尾 R signaling pathway can significantly stimulate the proliferation of NRK-49F cells and the transformation to myofibroblasts.
【作者单位】：青岛大学附属医院肾病科;山东大学齐鲁医院(青岛)泌尿内科;青岛大学附属医院中心实验室;
【基金】：青岛市科技计划基础研究项目[11-2-4-2-(12)-jch]~~
【分类号】：R692

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