CNPs对肾结石大鼠尿液NGAL、MCP-1水平和肾功能的影响

发布时间：2018-05-31 22:41

本文选题：钙化性纳米微粒 + 肾结石　；参考：《东南大学学报(医学版)》2017年05期

【摘要】：目的:探讨钙化性纳米微粒(CNPs)建立大鼠肾结石模型对其尿液中中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、单核细胞趋化蛋白-1(MCP-1)水平和肾功能的影响。方法:选取SPF级雄性SD大鼠24只,采用随机数字表法分为实验组、空白组各12只,实验组采取静脉注射CNPs建造大鼠肾结石模型,空白组注射等量生理盐水,检测实验后不同时间点两组大鼠尿液中NGAL、MCP-1的水平,结石形成情况,大鼠肾脏病理变化及肾功能变化。结果:实验组大鼠在鼠造模后4 h、12 h、24 h、1周、2周、8周尿液中NGAL、MCP-1水平呈现逐渐升高的趋势,并且每一个时间点对应的尿液NGAL水平均高于空白组(P0.05),造模后第1周、2周、8周时对应的尿液MCP-1水平均高于空白组(P0.05);实验组大鼠尿素氮、血肌酐水平在造模后第8周均高于空白组(P0.05);实验组大鼠肾脏病理检查均发现结石结晶形成,空白组未发现结石结晶形成。结论:CNPs建立大鼠肾结石模型的作用机制可能与升高NGAL、MCP-1水平有关,CNPs可造成肾脏功能的损伤。
[Abstract]:Aim: to investigate the effects of calcified CNPs) on the level of urinary neutrophil gelatinase-associated lipid carrier protein (NGAL1), monocyte chemoattractant protein (MCP-1) and renal function in rats with renal calculi. Methods: Twenty-four male SD rats of SPF grade were randomly divided into experimental group (n = 12) and blank group (n = 12). The experimental group was injected intravenously with CNPs to construct the model of renal calculi, and the blank group was injected with the same amount of normal saline. The levels of NGALL MCP-1 in urine, the formation of stones, the pathological changes of kidney and the changes of renal function in the two groups were measured at different time points after the experiment. Results: the levels of NGALL MCP-1 in urine of rats in the experimental group showed an increasing trend at 4 h, 12 h, 24 h, 1 week, 2 weeks and 8 weeks after the establishment of the model. The urine NGAL level of each time point was higher than that of the blank group, and the corresponding urine MCP-1 level at 2 weeks and 8 weeks after modeling was higher than that of the blank group, and the urea nitrogen in the experimental group was higher than that in the control group. The serum creatinine level was higher than that in the blank group at the 8th week after modeling, and the kidney pathology of the experimental group showed the formation of stone crystallization, but the blank group did not find the stone crystallization. Conclusion the mechanism of rat renal calculi induced by 10% CNPs may be related to the increase of NGALL MCP-1 level and the damage of renal function caused by CNPs.
【作者单位】：河南省中医院/河南中医药大学第二附属医院泌尿外科;
【分类号】：R692.4

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