去神经对大鼠前列腺组织中b-FGF表达的影响

发布时间：2018-06-03 17:34

本文选题：前列腺去神经 + 大鼠　；参考：《临床泌尿外科杂志》2013年11期

【摘要】：目的:探讨完全去前列腺神经后前列腺组织中碱性成纤维细胞生长因子(b-FGF)的表达对前列腺增生的影响。方法:将130只成年SD雄性大鼠随机分成四组,对照组10只,实验组120只,分别用0.01%戊巴比妥钠(1ml/kg)腹腔注射麻醉,实验组破坏支配前列腺的神经后缝合饲养,分别于术后1周,2周,3周各处死40只,完整取出前列腺,分别用于电镜与免疫组化检测。结果:实验组前列腺体积明显缩小,组织轻度水肿,无炎细胞浸润,HE染色与电镜下观察神经纤维显著减少,甚至消失,平滑肌逐渐变薄,腺细胞出现空泡变性,部分细胞器破坏,基底膜萎缩,胶原纤维轻度增生;b-FGF在各组均有表达,实验组随时间延长,表达逐渐减弱,与对照组比较差异有统计学意义(P0.05);术后1周与2周表达的差异无统计学意义,术后1、2周分别与3周比较,b-FGF表达的差异有统计学意义(P0.05)。结论:大鼠前列腺去神经后其体积缩小,前列腺组织中b-FGF的表达显著减弱。
[Abstract]:Aim: to investigate the effect of basic fibroblast growth factor (bFGF) on prostatic hyperplasia (BPH) after complete removal of prostatic nerve. Methods: 130 adult Sprague-Dawley rats were randomly divided into four groups: control group (n = 10) and experimental group (n = 120), which were anesthetized with 0.01% pentobarbital sodium (1 ml / kg) intraperitoneally respectively. Forty rats were killed at 1 week, 2 weeks and 3 weeks after operation. The prostate was removed completely and used for electron microscopy and immunohistochemistry respectively. Results: in the experimental group, the volume of prostate was obviously reduced, the tissue was slightly edema, the nerve fibers were decreased or even disappeared, the smooth muscle became thinner, and the gland cells showed vacuolation degeneration in the presence of HE staining without inflammatory cell infiltration and electron microscope. Some organelles were destroyed, basement membrane atrophied, collagen fibers were slightly proliferated and b-FGF was expressed in all groups, and the expression of b-FGF in experimental group decreased with time. There was no significant difference in the expression of b-FGF between 1 week and 2 weeks after operation, but there was a significant difference in the expression of b-FGF between 1 week and 2 weeks after operation compared with the control group (P 0.05). Conclusion: the volume of rat prostate was reduced and the expression of b-FGF was decreased significantly.
【作者单位】：遵义医学院附属医院泌尿外科;
【基金】：遵义市科技计划项目(编号200812)
【分类号】：R697.33

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