NGF和CgA对RWPE-1细胞内钙离子浓度变化的影响及其在慢性前列腺炎中的作用

发布时间：2018-06-06 16:54

  本文选题：前列腺炎 + 神经生长因子　； 参考：《安徽医科大学学报》2017年02期

【摘要】：目的探讨神经生长因子(NGF)和嗜铬颗粒蛋白A(CgA)处理后前列腺上皮细胞系RWPE-1细胞内钙离子浓度([Ca~(2+)]_i)的差异及NGF和CgA在慢性前列腺炎(CP)发病中的作用。方法不同浓度的NGF和CgA(50、200 ng/ml)刺激RWPE-1细胞,用激光共聚焦显微镜观察不同浓度的NGF和CgA刺激后细胞内[Ca~(2+)]_i变化,加入TRPV1受体拮抗剂后继续观察细胞内[Ca~(2+)]_i变化。然后,将正常细胞外液换成D-PBS,再加入NGF和CgA观察细胞内[Ca~(2+)]_i变化。结果 50 ng/ml NGF和CgA刺激细胞后,细胞内[Ca~(2+)]_i未见明显变化。200 ng/ml NGF和CgA刺激细胞后,细胞内[Ca~(2+)]_i明显增加,加入TRPV1受体拮抗剂后,细胞内[Ca~(2+)]_i未见明显变化。将正常细胞外液换成D-PBS,用NGF和CgA刺激后,细胞内[Ca~(2+)]_i未见明显改变。结论CP的发生可能是通过分泌和释放NGF和CgA,引起前列腺上皮细胞膜TRPV1通道开放,促使细胞内钙离子浓度增加,导致前列腺上皮分泌功能下降所致。
[Abstract]:Objective to investigate the difference of intracellular calcium concentration ([Caan2] I] in prostatic epithelial cell line RWPE-1 treated with NGF and CgA and the role of NGF and CGA in the pathogenesis of chronic prostatitis. Methods RWPE-1 cells were stimulated with different concentrations of NGF and CgA150 ng / ml. The changes of [Caan2] I in RWPE-1 cells were observed by confocal laser microscopy, and the intracellular [Caan2] I changes were observed after adding TRPV1 receptor antagonist to RWPE-1 cells. Then, the normal extracellular fluid was replaced with D-PBSand then NGF and CGA were added to observe the changes of [CaO2] I in the cells. Results after stimulation with 50 ng/ml NGF and CGA, no significant changes were observed in [Cajian2] in the cells. After stimulation with CGA and CGA, the intracellular [Cajian2] I was significantly increased, but no significant changes were observed in the [Cajian2] I after the addition of TRPV1 receptor antagonist. After the normal extracellular fluid was replaced with D-PBSand stimulated by NGF and CGA, the intracellular [Cajian2] had no obvious change. Conclusion CP may be caused by the secretion and release of NGF and CgA, which may lead to the opening of TRPV1 channel of prostate epithelial cell membrane and the increase of intracellular Ca ~ (2 +) concentration, resulting in the decrease of secretory function of prostatic epithelium.
【作者单位】： 安徽医科大学第一附属医院泌尿外科;安徽医科大学泌尿外科研究所;
【基金】：国家自然科学基金(编号:81170698、81400757) 安徽省自然科学基金(编号:1508085QH171) 卫生部国家临床重点专科建设项目(2012)
【分类号】：R697.33
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本文编号：1987443