溶瘤腺病毒联合放疗对膀胱肿瘤细胞的影响

发布时间：2018-06-07 00:44

本文选题：溶瘤腺病毒 + 膀胱肿瘤细胞　；参考：《兰州大学》2014年硕士论文

【摘要】：目的：探讨溶瘤腺病毒联合放疗对膀胱肿瘤细胞的影响。方法：使用CCK-8(cell counting kit-8)法检测对膀胱肿瘤细胞生存率的影响；流式细胞仪检测细胞周期和凋亡率；实时荧光定量PCR检测Bax、Bcl-2mRNA,自噬相关基因BECN、Atg-5mRNA, CAR mRNA表达情况；Western blot检测E1A、Bax、Bcl-2、细胞色素C蛋白表达情况。结果：联合治疗可以明显降低膀胱肿瘤细胞的生存率,呈剂量依赖性,并且具有协同作用；流式细胞仪检测显示联合治疗可以诱导膀胱肿瘤细胞周期阻滞并发生凋亡；实时荧光定量PCR检测显示联合治疗组Bax mRNA表达升高(p0.05),Bcl-2mRNA表达下降(p0.05)；自噬相关基因BECN, Atg-5mRNA与对照组相比表达下降(p0.05)；联合组与单纯作用组相比CAR mRNA表达水平是增高的(p0.05)；Western blot检测显示实验组E1A蛋白、Bax、细胞质中细胞色素C增高,Bcl-2、线粒体中细胞色素C降低。结论：联合治疗组对肿瘤细胞杀伤具有协同效应有可能是通过线粒体凋亡通路来诱导膀胱肿瘤细胞发生凋亡,从而发挥抑制肿瘤增殖效应。
[Abstract]:Objective: to investigate the effect of adenolysin combined with radiotherapy on bladder tumor cells. Methods: CCK-8(cell counting kit-8 method was used to detect the survival rate of bladder tumor cells, flow cytometry was used to detect cell cycle and apoptosis rate. Baxan Bcl-2 mRNAs were detected by real-time fluorescence quantitative PCR, autophagy related gene BECN-5mRNAs, CAR mRNA expression was detected by Western blot, and cytochrome C protein expression was detected by Western blot. Results: combined therapy could significantly reduce the survival rate of bladder tumor cells in a dose-dependent manner and had synergistic effects. Flow cytometry showed that combined therapy could induce cell cycle arrest and apoptosis of bladder tumor cells. Real-time fluorescence quantitative PCR analysis showed that the expression of Bax mRNA increased in the combined treatment group and decreased in the combination group. The expression of autophagy related gene BECNN, Atg-5mRNA was lower than that of the control group, and the expression level of CAR mRNA in the combined group was higher than that in the control group. The expression level of CAR mRNA in the combined group was higher than that in the control group, and the expression level of CAR mRNA in the combined group was higher than that in the control group. The results showed that cytochrome C in cytochrome C increased and cytochrome C in mitochondria decreased in the experimental group. Conclusion: the synergistic effect of combined treatment group on tumor cell killing may be induced by mitochondrial apoptosis pathway to induce apoptosis of bladder tumor cells and thus play a role in inhibiting tumor proliferation.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.14

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