C肽对糖尿病肾病大鼠肾小管间质氧化应激的影响

发布时间：2018-06-08 06:42

本文选题：糖尿病肾病 + C肽　；参考：《实用医学杂志》2017年09期

【摘要】：目的:探讨C肽对链脲佐菌素诱导的糖尿病肾病大鼠肾小管间质氧化应激的影响。方法:腹腔注射链脲佐菌素的Wistar大鼠发展至糖尿病肾病后,随机分为对照组、C肽组,经腹腔埋入微渗泵用药。以同龄正常Wistar大鼠为正常组。用药前及用药后每4周检测各组大鼠血糖及24 h尿白蛋白。用药12周后,免疫组织化学染色观察大鼠肾小管间质转化生长因子β1(TGF-β1)表达;新鲜肾小管间质组织匀浆WST-1法检测超氧化物歧化酶(SOD)活性,TBA法检测丙二醛(MDA)含量,Real-time PCR检测蛋白激酶A m RNA的表达。结果:与正常组对比,对照组大鼠血糖及24 h尿白蛋白升高,肾小管间质TGF-β1表达增加、SOD活性降低、MDA含量升高、蛋白激酶A m RNA表达下调;经C肽干预后,大鼠血糖无明显变化,24 h尿白蛋白升高减缓,肾小管间质TGF-β1表达减少、SOD活性升高、MDA含量降低、蛋白激酶A m RNA表达上调。结论:C肽通过激活蛋白激酶A途径减轻糖尿病肾病大鼠肾小管间质的氧化应激反应,改善肾小管间质纤维化,减轻蛋白尿。
[Abstract]:Aim: to investigate the effect of C-peptide on renal tubulointerstitial oxidative stress induced by streptozotocin in diabetic nephropathy rats. Methods: Wistar rats with streptozotocin were injected intraperitoneally to diabetic nephropathy and were randomly divided into control group and C peptide group. Normal Wistar rats of the same age were used as normal group. Blood glucose and 24 h urinary albumin were measured before and every 4 weeks after administration. After 12 weeks of administration, the expression of TGF- 尾 1 in renal tubulointerstitial tissue was observed by immunohistochemical staining. The activity of superoxide dismutase (SOD) in fresh renal tubulointerstitial tissue homogenate was detected by WST-1 method and the content of malondialdehyde (MDA) MDAs was detected by TBA method. Protein kinase A mRNA expression was detected by Real-time PCR. Results: compared with the control group, the blood glucose and 24 h urinary albumin in the control group were increased, the expression of TGF- 尾 1 in the renal tubulointerstitium was increased, the activity of SOD was decreased, the expression of protein kinase A mRNA was decreased, and the expression of protein kinase A mRNA was down-regulated after the intervention of C-peptide. There was no significant change in blood glucose in rats. The increase of urinary albumin decreased, the expression of TGF- 尾 1 in renal tubulointerstitium decreased, the activity of SOD increased and the content of MDA decreased, and the expression of protein kinase A mRNA increased. Conclusion the oxidative stress response of renal tubulointerstitial in diabetic nephropathy rats was alleviated by activating protein kinase A pathway, tubulointerstitial fibrosis was ameliorated, and proteinuria was alleviated.
【作者单位】：中山大学附属第五医院;
【基金】：广东省医学科研基金项目(编号:B2014168) 珠海市科技计划医疗卫生项目(编号:2015A1003)
【分类号】：R587.2;R692.9

【相似文献】