肾组织干细胞治疗急性肾衰竭的可行性

发布时间：2018-06-13 05:38

本文选题：干细胞 + 肾小管坏死　；参考：《中国组织工程研究》2017年01期

【摘要】：背景:研究表明,肾组织干细胞主要存在于肾乳头部位,其具有类似于间充质干细胞的特性,目前尚不能肯定其是否参与了肾小管上皮细胞损伤的修复。目的:探讨肾组织干细胞治疗急性肾衰竭的效果及可行性。方法:纳入40只SD大鼠,10只用于肾组织干细胞培养,其余30只随机分为3组,实验组和对照组制备急性肾衰竭动物模型,正常组不做任何处理。模型制备后6 h,实验组尾静脉注入肾组织干细胞,对照组注入相同剂量的生理盐水,正常组不做任何处理。移植后10 d检测肾功能,获取肾组织标本进行组织学观察。结果与结论:(1)对照组的尿素氮、肌酐水平均显著高于正常组和实验组(P0.05);实验组与正常组之间差异无显著性意义(P0.05);(2)正常组皮质和外髓质小管未出现异常改变,无炎性浸润;对照组外髓质小管萎缩,炎症细胞浸润,上皮细胞肿胀;实验组有轻微炎症细胞浸润和细胞肿胀等病变,但较对照组明显较轻;(3)结果表明,肾组织干细胞移植能有效改善急性肾衰竭大鼠的肾功能,促进上皮细胞再生,达到良好的组织修复效果。
[Abstract]:Background: studies have shown that renal stem cells mainly exist in the site of renal papilla, which is similar to mesenchymal stem cells. At present, it is not certain whether renal stem cells are involved in the repair of renal tubular epithelial cell injury. Objective: to investigate the efficacy and feasibility of renal stem cells in the treatment of acute renal failure. Methods: ten SD rats were used in renal stem cell culture. The other 30 rats were randomly divided into 3 groups. The experimental group and the control group were used to make acute renal failure animal model, the normal group did not do any treatment. Six hours after the model was made, renal stem cells were injected into the tail vein of the experimental group, and the same dose of normal saline was injected into the control group, but no treatment was done in the normal group. Renal function was detected 10 days after transplantation. Results and conclusion the levels of urea nitrogen and creatinine in the control group were significantly higher than those in the normal group and the experimental group (P0.05), but there was no significant difference between the experimental group and the normal group (P 0.05) the cortical and outer medullary tubules in the normal group had no abnormal changes and no inflammatory infiltration. In the control group, the medullary tubule atrophy, inflammatory cell infiltration, epithelial cell swelling, slight inflammatory cell infiltration and cell swelling were found in the experimental group, but were significantly lighter than those in the control group. Renal stem cell transplantation can effectively improve the renal function of rats with acute renal failure, promote the regeneration of epithelial cells, and achieve a good tissue repair effect.
【作者单位】：南阳市中心医院泌尿外科;
【分类号】：R692.5

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