直接肾素抑制剂阿利吉仑对肾纤维化小鼠上皮-间质转化的调节作用

发布时间：2018-06-14 23:35

本文选题：阿利吉仑 + 肾纤维化　；参考：《吉林大学学报(医学版)》2017年01期

【摘要】：目的:探讨直接肾素抑制剂阿利吉仑对单侧输尿管结扎引起的小鼠肾纤维化中上皮-间质转化(EMT)的调节作用,阐明阿利吉仑抗肾纤维化的作用机制。方法:24只雄性ICR小鼠分为假手术组、模型组和阿利吉仑干预组,采用单侧输尿管结扎方法制作小鼠肾纤维化模型。HE染色观察小鼠肾间质纤维化的病理组织形态,Masson染色观察小鼠肾间质纤维化中胶原蛋白的表达情况,免疫组织化学染色法观察胞浆中α平滑肌肌动蛋白(α-SMA)的表达,免疫印迹法检测小鼠肾组织中E钙黏蛋白(E-cad)、α-SMA和Ⅰ型胶原(ColⅠ)的表达水平。结果:通过单侧输尿管结扎方法成功制备小鼠肾纤维化模型。HE染色和Masson染色,与模型组比较,阿利吉仑干预组小鼠肾纤维化程度明显降低,胶原蛋白表达明显减少。免疫组织化学染色,阿利吉仑干预组小鼠肾小管间质纤维化区域胞浆内α-SMA阳性显色较模型组明显减少。免疫印迹法,模型组小鼠肾组织中α-SMA和ColⅠ蛋白表达水平升高,分别是假手术组的1.79倍和1.95倍;E-cad蛋白表达水平降低,为假手术组的37.23%,组间比较差异有统计学意义(P0.05);阿利吉仑干预组小鼠肾组织中α-SMA和ColⅠ蛋白表达水平降低,E-cad蛋白表达水平升高,分别是模型组的63.72%、65.54%和2.1倍,组间比较差异均有统计学意义(P0.05)。结论:阿利吉仑能够通过上调E-cad的表达同时下调α-SMA、ColⅠ的表达抑制小鼠肾纤维化中EMT,进而发挥抗纤维化的作用。
[Abstract]:Objective: To investigate the regulatory effect of direct renin inhibitor alisiren on the epithelial mesenchymal transition (EMT) in renal fibrosis induced by unilateral ureteral ligature in mice, and to elucidate the mechanism of the action of alisjun on renal fibrosis. Methods: 24 male ICR mice were divided into sham operation group, model group and aligilam intervention group with unilateral ureteral ligation prescription. The pathological tissue morphology of renal interstitial fibrosis was observed by.HE staining in mice. The expression of collagen in renal interstitial fibrosis in mice was observed by Masson staining. The expression of alpha smooth muscle actin (alpha -SMA) in the cytoplasm was observed by immunohistochemical staining. E calcium sticky eggs in mouse kidney tissues were detected by immunoblotting. The expression level of white (E-cad), alpha -SMA and type I collagen (Col I). Results: the mouse renal fibrosis model.HE staining and Masson staining were successfully prepared by unilateral ureteral ligature. Compared with the model group, the degree of renal fibrosis was significantly reduced in the aligilam intervention group and the expression of collagen protein was significantly reduced. The cytoplasmic alpha -SMA positive color in the renal tubulointerstitial fibrosis area of the mice was significantly lower than that in the model group. The level of the expression of alpha -SMA and Col I protein in the renal tissue of the model group was 1.79 times and 1.95 times that of the sham group, and the expression level of E-cad protein decreased, which was 37.23% of the sham operation group, and the difference was different between the groups. There was statistical significance (P0.05); the expression level of alpha -SMA and Col I protein in the renal tissue of the aligilam intervention group decreased and the expression level of E-cad protein increased, which were 63.72%, 65.54% and 2.1 times of the model group, respectively, and the differences were statistically significant (P0.05). Conclusion: alirun can down regulate the expression of E-cad and reduce the alpha -SMA, Col The expression of EMT inhibits renal fibrosis in mice, and thus plays an anti fibrotic role.
【作者单位】：中国医科大学附属第一医院儿科;
【基金】：高等学校博士学科点专项科研基金资助课题(20122104110001) 辽宁省沈阳市科技局科技计划项目资助课题(F16-206-9-44)
【分类号】：R692

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